PPAR? Ligand Screening Assay Kit

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Cat No: 10007685

Assay Kits - Fluorometric Assays

Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: α, β (also called δ and NUC1), and γ. PPARγ is the most widely studied PPAR and exists in two protein isoforms (γ1 an...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

FP-based peroxisomes proliferator-activated receptors PPARs proliferator activated PPARgamma PPAR.gamma. PPARg PPAR? PPAR-? PPAR-gamma gamma g ? .gamma. PPAR-.gamma. PPAR-g kits assys atherosclerosis TF diabetes drugs discovery discover endocrinology high throughput screens screening inflammations inhibitors inhibits inhibition metabolic syndromes metabolism nuclear receptors fluorescence FP-based FP based base ligands probes proteins isoforms ?1 ?2 ?-1 ?-2 1 2 adipose tissues colon immune systems retinas adipogenesis cells cellular differentiation insulin sensitization cancers fatty acids FAAH arachdionic 15-deoxy-DELTA12,14-prostaglandin 15-deoxy-DELTA12,14-PGs 15-deoxy-DELTA12,14-PGJ2 15 deoxy DELTA 12,14 PGs prostaglandins PGJ2 15-deoxy-?12,14-PGs 15-deoxy-?12,14-prostaglandins 15-deoxy-?12,14-PGJ2 15-deoxy-D12,14-prostaglandins 15-deoxy-D12,14-PGs 15-deoxy-D12,14-PGJ2 15-deoxy-.DELTA.12,14-prostaglandins 15-deoxy-.DELTA.12,14-PGs 15-deoxy-.DELTA.12,14-PGJ2 J2 thiazolidinediones TZD pioglitazone rosiglitazone agonists hyperglycemia hyperinsulinemia hypertriglyceridemia types 2 antidiabetics diabetes oral antagonists

Product Overview:
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: α, β (also called δ and NUC1), and γ. PPARγ is the most widely studied PPAR and exists in two protein isoforms (γ1 and γ2) due to use of an alternative promoter and alternative splicing.{12922} PPARγ is primarily expressed in adipose tissue and to a lesser extent in colon, the immune system, and the retina.{12196} PPARγ was first identified as a regulator of adipogenesis, but also plays an important role in cellular differentiation, insulin sensitization, atherosclerosis, and cancer. Ligands for PPARγ include fatty acids, arachidonic acid metabolites such as 15-deoxy-D12,14-PGJ2, as well as the thiazolidinedione class of compounds (TZD) which include pioglitazone and rosiglitazone.{12961} TZDs are potent, selective PPARγ agonists that lower the hyperglycemia, hyperinsulinemia, and hypertriglyceridemia found in type 2 diabetic subjects and are presently used as oral antidiabetic drugs.{7654,10857} The use of these synthetic ligands has increased the understanding of PPARγ’s mechanism of activation and subsequent biological effects. By increasing our understanding of PPARγ additional drug candidates may be identified. Cayman’s PPARγ Ligand Screening Assay Kit provides a convenient fluorescence polarization (FP)-based single step assay for screening PPARγ ligands. In this assay, a ligand of PPARγ was conjugated to fluorescein and is used as the displacement probe. Ligands, agonists, and antagonists of PPARγ will displace the fluorescent probe leading to a decrease in FP. The PPARγ Ligand Screening Assay Kit is a robust assay with a Z’ of 0.81 and has a dynamic range of greater than 120 mP units. The assay has been validated using known agonists/ligands of PPARγ (Arachidonic Acid, Rosiglitazone, Troglitazone, etc.) with IC50 values ranging from nanomolar to millimolar concentrations.

Size	384 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.