PH Domain Leucine-rich Repeat Pr

Confirm delete?

PH Domain Leucine-rich Repeat Protein Phosphatase 1 Polyclonal Antibody

Zoom

Cat No: 10007191

Cayman’s PHLPP1 Polyclonal Antibody detects the carboxy-terminal amino acids within the PDZ-binding domain of both the PHLPP1 α and β isoforms. PHLPP2 shares partial identity with the domains of PHLPP1 however it has a unique PDZ-binding domain sequen...

This product can only be bought through Cayman Chemical. Please contact us.

Product Overview
Data
Downloads
About Cayman

Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

h-SCOP PHLPP KIAA 0606 PLEKHE ICC WB IHC

Product Overview:
Cayman’s PHLPP1 Polyclonal Antibody detects the carboxy-terminal amino acids within the PDZ-binding domain of both the PHLPP1 α and β isoforms. PHLPP2 shares partial identity with the domains of PHLPP1 however it has a unique PDZ-binding domain sequence. Protein kinase B/Akt is critical in regulating cell growth and death. Activation of a serine residue (Ser473) in a C-terminal hydrophobic motif of Akt is known to be linked to some of the most common human cancers. PHLPP1 and PHLPP2 dephosphorylate the hydrophobic motif of Akt and thus reduce Akt activity, resulting in a increase in the number of apoptotic cells.{13717} PHLPP1 levels are markedly reduced in several colon cancer and glioblastoma cell lines that have elevated Akt phosphorylation. Injection of a human glioblastoma cell line overexpressing PHLPP1 into mice reduced tumor size by nearly 70% compared with control animals. mRNA of PHLPP1 is ubiquitously expressed, with highest levels found in brain.{13717} Cayman’s PHLPP Polyclonal Antibody detects the enzyme at around 150 kDa (α isoform) and 200 kDa (β isoform) by western blot from various cell lines (THP-1) and tissues such as brain.

Size	1 ea
Shipping	dry ice
Host	Rabbit
Antigen	Synthetic peptide from the C-terminal region of human PHLPP1 (? isoform)
Application(s)	ICC, IHC (paraffin-embedded tissue), and WB
Formulation	Peptide affinity-purified antibody lyophilized from 500 ?l
Custom Code	3822.19
UNSPSC code	12352203

Technical File

MSDS

Click here to ask for your quote and get 15% off Cayman's products.

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.