Lipase Activity Assay Kit

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Cat No: 700640

Assay Kits - Enzyme Activity

Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic d...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

assays kits measures measurements lipases digestions transportations dietary plasmas triglycerides levels coronary hearts diseases CHD blood HDLs high density lipoproteins low VLDLs synthesis degradations very chylomicron particles triglyceride-rich LPLs hepatic HL interlipoproteins exchanges cholesteryl esters transfers proteins hydrolysis hydrolyzing phospholipases metabolisms conversions adipocytes skeletal muscles cells cardiac livers endothelial ELs vascular macrophages smooth A1 PLA1 HDL-phospholipids sn-1 HDL-C inflammation atherosclerosis triacylglycerols adipose fat obese tissues hydrolytics hormones hormone-sensitives monoacylglycerides MAGLs ATGLs fatty acids diacylglycerides hydrolyzed glycerols lipolysis energy metabolic disorders obesity therapeutics therapies weight inhibits inhibition inhibitors gastrointestinal absorption colipases colipase-dependent pancreatic pancreas carboxy esters enzymes catalysis bonds orlistat gastric fluorescence-based homogenates cultures supernatants fluorescent

Product Overview:
Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity. Cayman’s Lipase Activity Assay provides a fluorescence-based method for detecting lipase activity in plasma, serum, tissue homogenates, and cell culture samples. In the assay, lipase hydrolyzes arachidonoyl-1-thioglycerol to arachidonic acid and thioglycerol. Thioglycerol reacts with the thiol fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 380-390 nm and an emission wavelength of 510-520 nm.

Size	96 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.