Myeloperoxidase Inhibitor Screen

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Myeloperoxidase Inhibitor Screening Assay Kit

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Cat No: 700170

Assay Kits - Fluorometric Assays

Cayman's MPO Inhibitor Screening Assay Kit provides convenient fluorescence-based method for identifying novel inhibitors of the chlorination and peroxidation activities of myeloperoxidase (MPO). The chlorination assay uses the non-fluorescence APF. A...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

heme peroxidases azurephilic granules leukocytes neutrophils monocytes tissues macrophages chlorides hydrogen peroxides H2O2 Cl- hypochlorous acids acute chronic vascular inflammatory diseases cardiac therapeutics cycles fluorescence fluorescent assays kits measures measurement crude cells lysates purified enzymes ADHP 10-acetyl-3,7-dihydroxyphenoxazine resorufin 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid APF -OCl assays

Product Overview:
Cayman's MPO Inhibitor Screening Assay Kit provides convenient fluorescence-based method for identifying novel inhibitors of the chlorination and peroxidation activities of myeloperoxidase (MPO). The chlorination assay uses the non-fluorescence APF. APF is selectively cleaved by hypochlorite (-OCl) to yield free fluorescein. which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 480-495 and 515-525 nm, respectively. The peroxidation assay is based on the two-electron oxidation of native MPO to MPO-I by hydrogen peroxide, followed by two successive one-electron reductions back to MPO by ADHP with an MPO-II intermediate. The reaction between hydrogen peroxide and ADHP yields resorufin, which can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively.

Size	2 x 96 wells
Shipping	wet ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.