CBP bromodomain TR-FRET Assay Ki

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CBP bromodomain TR-FRET Assay Kit

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Cat No: 600850

Assay Kits - Fluorometric Assays

Cayman’s CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

TRFRET BRDEu BRDEu3+ HTS

Product Overview:
Cayman’s CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of CBP bromodomain (BRD; human; amino acids 1,081-1,197) directly labeled with a europium (Eu3+) chelate (BRD-Eu3+). A biotinylated peptide containing acetylated lysines serves as the ligand for the CBP bromodomain. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-labeled avidin resulting in a loss of TR-FRET signal. The CBP bromodomain TR-FRET Assay Kit is robust (Z´ = >0.6) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.

Size	384 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.