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NAD/NADH Cell-Based Assay Kit

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    NAD/NADH Cell-Based Assay Kit
  • NAD/NADH Cell-Based Assay Kit
Cat No: 600480
Assay Kits - Cell-Based Assays
Cayman

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making ...

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • cells assay kits CBAs colorimetric measuring measurement Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide reduced cells oxidized cofactors redox oxidations reductions reactions electrons transporters cellular substrates enzymes acetyl groups proteins posttranslational respiratory functions citric acids cycles tricarboxylic TCA Kreb's ATP adenosine 5'-triphosphate oxidative phosphorylation metabolic activity biosynthesis de novo phosphoribosyltransferase NAMPT organs aging cytosolic mammalian death apoptosis signaling transcriptional metabolic disorders intracellular cultured alcohol dehydrogenases acetoaldehydes NAD kit NADP NADH NAD+ NAD-+ +
Product Overview:
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric method for measuring intracellular NAD+ and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.
Size 1 ea
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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