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Autophagy/Cytotoxicity Dual Staining Kit

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    Autophagy/<wbr/>Cytotoxicity Dual Staining Kit
  • Autophagy/<wbr/>Cytotoxicity Dual Staining Kit
Cat No: 600140
Assay Kits - Cell-Based Assays
Cayman

Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accu...

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • assays kits measuring measurement MDC cell-based autophagy cells cytotoxicity staining autofluorescent autofluorescence substances multilamellar body bodies ions trapping mechanisms membranes lipids probes autophagic vacuoles cultured degradation digestion intracellular lysosomes organelles misfolded proteins microorganisms cytoplasms double membranes autophagosomes autolysosomes homeostasis dysregulation cancers infections aging degenerative diseases stress death apoptosis
Product Overview:
Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms.{17131} Autophagy is a multi-step process that begins with the sequestration of cytoplasm, organelles, and proteins. These cellular components are sequestered by a double membrane, forming an autophagosome. The autophagosome then fuses with a lysosome to form an autolysosome, where the cellular material is then degraded.{15627,17132} Normal autophagy is essential for survival, differentiation, development, and homeostasis. Dysregulation of autophagy has been implicated in cancer, infection, aging, and degenerative diseases.{15627} While autophagy most often acts to promote cell survival in response to stress, it can also promote cell death. The relationship between autophagy and apoptosis is complex. The two pathways share common stimuli, components, and can regulate the activity of each other.{17133} However, the specific factors and mechanisms that dictate the choice between autophagy and apoptosis remain unclear. Cayman’s Autophagy/Cytotoxicity Dual Staining Kit provides a convenient tool for studying the regulation of autophagy and cytotoxicity at the cellular level. The kit employs monodansylcadaverine (MDC), an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and the interaction with membrane lipids,{17214} as a probe for detection of autophagic vacuoles in cultured cells. Propidium iodide is used as a marker of cell death. Tamoxifen, a known inducer of autophagy, is included as a positive control.
Size 1 ea
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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