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PAD4 (human, recombinant; His- and GST-tagged)

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    PAD4 (human, recombinant; His- and GST-tagged)
  • PAD4 (human, recombinant; His- and GST-tagged)
Cat No: 25915
Proteins - Enzymes

Protein arginine deiminase 4 (PAD4) catalyzes the conversion of arginine residues to citrulline within cellular protein substrates, resulting in the loss of a positive charge, which can alter protein structure and/or function.{45610} It is expressed i...

: 100 µg

This product can only be bought through Cayman Chemical. Please contact us.

Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • PAD-1 4 6 EC 3.5.15 EC3.5.15 HL-60 HL60 PADIH PADI-H PDI4 PTM RA MS Escherichia PDI5
Product Overview:
Protein arginine deiminase 4 (PAD4) catalyzes the conversion of arginine residues to citrulline within cellular protein substrates, resulting in the loss of a positive charge, which can alter protein structure and/or function.{45610} It is expressed in neutrophils, as well as a variety of tissues, including the brain, liver, lung, and kidney.{45610,45611,18134} PAD4 has a key role in NETosis, a lytic form of cell death characterized by the release of neutrophil extracellular traps (NETs).{45610} Upon neutrophil activation, PAD4 translocates to the nucleus where it citrullinates histones, initiating chromatin decondensation and the release of NETs.{45611,19778,61223} Neutrophils isolated from Pad4-/- mice exhibit decreased citrullination of histone H3 under both basal and LPS-stimulated conditions and are defective for NET formation in response to stimulation with LPS, phorbol 12-myristate 13-acetate (PMA; Item No. 10008014), or hydrogen peroxide.{19778} Pad4-/- mice exhibit larger lesions than wild-type mice in a model of necrotizing fasciitis induced by M1 group A S. pyogenes lacking the extracellular DNase Sda1. Pad4-deficient mice also exhibit reduced infarct size in a model of myocardial ischemia-reperfusion injury and reduced tumor growth in a Lewis lung carcinoma model.{45612,45611} Serum PAD4 autoantibodies have been found in patients with rheumatoid arthritis and are associated with disease severity.{19300} Cayman’s PAD4 (human, recombinant; His- and GST-tagged) protein can be used for enzyme activity assays. This protein contains two types of affinity tags, His and GST, that allow for flexibility in the removal of PAD4 following citrullination reactions and an enterokinase cleavage site that facilitates removal of both the His and GST tags.
Size 100 µg
Shipping dry ice
Formulation 50 mM HEPES, pH 8.0, 300 mM sodium chloride, 1mM DTT, and 10% glycerol
Purity ≥85% estimated by SDS-PAGE
Custom Code 3504.00
UNSPSC code 12352204

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Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

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