€593.00 €504.05
Myeloid cell leukemia-1 (Mcl-1) is a Bcl-2 family member protein that is involved in the regulation of apoptosis.{64165} Alternative splicing of MCL1 pre-mRNA produces three isoforms, anti-apoptotic Mcl-1 long (Mcl-1L) and pro-apoptotic Mcl-1 short and Mcl-1 extra short (Mcl-1S and Mcl-1ES, respectively). Mcl-1L is composed of a C-terminal helix that acts as a membrane anchor, four Bcl-2 homology (BH) domains, and a PEST domain. The BH1, BH2, and BH3 domains facilitate heterodimerization with pro-apoptotic proteins, including Bax and Bak, and homodimerization with Mcl-1S and Mcl-1ES.{64165,64166,64167} Mcl-1L is expressed during embryogenesis and hematopoiesis, as well as during nervous and immune system development, and localizes to the mitochondrial membrane.{55182,64168} It inhibits apoptosis by preventing Bax and Bak oligomerization, which form a pore on the outer mitochondrial membrane that allows release of cytochrome c into the cytoplasm.{64165} Knockdown of MCL1 sensitizes oral cancer cells to cisplatin and tumor levels of Mcl-1L are increased relative to adjacent normal tissues in patients with oral cancer.{64169} Overexpression of MCL1 is associated with poor prognosis in several cancers, including solid tumors and hematological malignancies.{64165} Cayman’s Mcl-1L Cytoplasmic Domain (human, recombinant) protein can be used for enzyme activity assays.
Territorial Availability: Available through Bertin Technologies only in France
| Size | 100 µg |
|---|---|
| Shipping | dry ice |
| Molecular weight | 0 |
| Formulation | 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 0.04% Tween 20, 3 mM DTT, 20% glycerol, and 200 mM imidazole |
| Purity | ≥90% estimated by SDS-PAGE |
| Custom code | 3504.00 |
| UNSPSC code | 12352204 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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