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AFABP (human) ELISA kit

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    AFABP (human) ELISA kit
  • AFABP (human) ELISA kit
Cat No: A05181
Assays - Hrp Kits
SPI-Bio
€682.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Adipocyte Fatty Acid Binding Protein (AFABP) is a 15 kDa member of the intracellular Fatty Acid Binding Protein (FABP) family, which is known for the ability to bind fatty acids and related compounds.
AFABP is expressed in a tissue specific-manner in differentiated adipocytes and is a critical gene in the regulation of the biological function of these cells. According to some results, AFABP may be involved in mediating obesity-induced alterations in adipocyte gene expression. In macrophages AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages. Besides being active within the cell, AFAB appears to be a secreted protein, with unknown functions.
Size: 96 wells
Shipping: Wet ice
Stability: Store at +4degrees; shelf life 6 months maximum after production
Application Media: Human serum, plasma, tissue extract and culture supernatant|1/10 dilution for serum and plasma prior assay
Sample volume: 20 µL
Tracer: HRP
Detection Limit: 0.1 ng/mL
Standard Curve Range: 0.5-25 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

Gender differences in factors influencing insulin resistance in elderly hyperlipemic non-diabetic subjects. Lichnovska R, Gwozdziewiczova S, Hrebicek J. Cardiovasc Diabetol; Oct 14;1(1):4 (2002)

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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