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IL-28B (pig) ELISA kit

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    IL-28B (pig) ELISA kit
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  • IL-28B (pig) ELISA kit
Cat No: A05425
€395.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 28B (IL-28B), recently renamed interferon- λ3 (IFNλ3), is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29). It has been classified as type III interferon(2).
IL-28B is a polypeptide of 170 amino acids with a molecular weight of 19.6 kDa. IL-28B structure contains six antiparallel helices(3)
This cytokine is produced at a monomeric state(4) by plasmacytoid dendritic cells after virus infection or dsRNA. Moreover its production is enhanced by Interferon-α.
The main function of IL28B is antiviral action by the modulation of the expression of MHC (Type I/II) in dentritic cells or by the modulation of CCR7 receptor mediating dentritic cells migration.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,02 ng/mL
Standard Curve Range: 0,02-2,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

1. Sabat R IL-10 family of cytokines 2010, Cytokine Growth Factor Rev 21 : 315–324

2. Zdanov A (2010) Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21 : 325–330

3. Robert Sabat, Ellen Witte, Katrin Witte, a