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VEGF-A (pig) ELISA kit

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    VEGF-A (pig) ELISA kit
  • VEGF-A (pig) ELISA kit
Cat No: A05426
Assays - Hrp Kits
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Vascular endothelial growth factor (VEGF-A or VEGF)(1), also known as vascular permeability factor (VPF)(2), has been discovered in 1983. It is a member of the VEGF family that includes in mammals placental growth factor (PLGF), VEGF-B, VEGF-C, and VEGF-D (also known as c-Fos-induced growth factor, FIGF)(1).
VEGF-A is a glycoprotein having several isoforms of 121 to 206 amino acids(1) with a molecular weight from 33 kDa to 57kDa. It is a homodimer stabilised by hydrophobic interactions and by a disulphide bound(3). The monomer structure contains a central antiparallel four-stranded β sheet with a cystine knot at the end (4).
VEGF-A is produced by many cells like fetal tissu, hematopoietic stem cells, epidermal keratinocytes, podocytes of the renal glomerulus, cardiac myocytes, prostatic epithelium, and certain epithelial cells of the adrenal cortex and lung(5).
The main function of the VEGF-A is the angionesis (formation of new capillaries from pre-existing vessels) during the embryogenesis(1) or during re-building of the blood vessel after an injury. It has also some secondary functions in the chemotacxis of macrophages/ granulocytes and in vasodilation (indirectly by NO release).
Due to its main function, overexpression of VEGF-A causes various diseases including cancer.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,01 ng/mL
Standard Curve Range: 0,01-1,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

1. David IR Holmes and Ian Zachary The vascular endothelial growth factor (VEGF) family: angiogenic factors in health and disease 2005, Genome Biology, 6 :209

2. Angela M. Duffy, David J. Bouchier-Hayes, and Judith H. Harmey Vascular Endothelial Growth Factor (VEGF) and Its Role in Non-Endothelial Cells: Autocrine Signalling by VEGF 2000-2013, Madame Curie Bioscience Database

3. Christopher J. Robinson and Sally E. Stringer The splice variants of vascular endothelial growth factor (VEGF) and their receptors Journal of Cell Science 114, 853-865

4. Yves A Muller, Hans W Christinger, Bruce A Keyt, Abraham M de Vos The crystal structure of vascular endothelial growth factor (VEGF) refined to 1.93 Å resolution: multiple copy flexibility and receptor binding 1997, Structure Volume 5, Issue 10, 1325-1338

5. Dvorak HF, Brown LF, Detmar M, Dvorak AM Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. 1995, Am J Pathol, 146:1029-1039

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.