TNF-alpha (pig) ELISA kit

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Cat No: A05411

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Cachectin

Tumor Necrosis Factor-alpha

Correlated keywords:

ELISA

Interleukin-1

Interleukin-6

Cytokine

Inflammatory

Chemokines

Swine

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Known as cachectin, the TNF-alpha (Tumor Necrosis Factor-alpha) is a glycoprotein of 156 amino acids produced from a precursor processed by action of a metalloprotease. Isolated for the first time in 1975 by Carswell, it’s a cytokine of the innate immunity.
Interleukin-1 or bacterial endotoxins stimulate the production of TNF-alpha by immune cells (activated macrophages, NK cells, monocytes) and non-immune cells (keratinocytes, vascular smooth muscle cells, and granulosa lutein cells).
The main function of the TNF-alpha is to act as an immunomodulator and as a mediator of infl ammatory responses in the phenomena of host resistance to infection. It allows neutrophils and macrophages recruitment to infectious sites by stimulating the production of adhesion molecules and chemokines (chemotactic cytokines) by endothelial cells. TNFalpha also acts on the hypothalamus to trigger the fever.
Over-production of TNF-alpha has been implicated in a number of pathological conditions including cachexia, septic shock, and autoimmune disorders.

Size	96 wells
Shipping	wet ice
Specificity	refer to technical booklet
Application Media	It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume	100 µL
Tracer	horseradish peroxidase HRP
Detection Limit	≤0,02 ng/mL
Standard Curve Range	0,02-2,00 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

Carswell E. A., Old L. J., Kassel R. L. et al. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA (1975) 72:3666-70

Trebichavský I., Splíchal I., Barot-Ciorbaru R. Expression of TNF-alpha in pig fetal cells stimulated in vitro. Folia Microbiol (Praha).1995;40(4):417-20

Allen D.G., Riviere J.E., Monteiro-Riviere N.A. Cytokine induction as a measure of cutaneous toxicity in primary and immortalized porcine keratinocytes exposed to jet fuels, and their relationship to normal human epidermal keratinocytes. Toxicol Lett. 2001 Mar 8;119(3):209-17

Newman W.H., Zhang L.M., Lee D.H. et al. Release of tumor necrosis factor-alpha from coronary smooth muscle: activation of NF-kappaB and inhibition by elevated cyclic AMP. J Surg Res. 1998 Dec;80(2):129-35

Vézina S. A., Roberge D., Fournier M. et al. Cloning of porcine cytokine-specifi c cDNAs and detection of porcine tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-1 beta gene expression by reverse transcription PCR and chemiluminescence hybridization. Clin Diagn Lab Immunol. 1995 Nov;2(6):665-71

Sundgren-Andersson A.K., Ostlund P., Bartfai T. IL-6 is essential in TNF-alpha-induced fever. Am J Physiol. 1998 Dec;275(6 Pt 2):R2028-34

Vilcek J., Lee T.H. Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions J Biol Chem 1991 Apr 25;266(12):7313-6

Ware C.F. et al Apoptosis mediated by the TNF-related cytokine and receptor families. J. Cellular Biochemistry 1996 60:47

Aggarwal B.B., Vilcek J., eds Tumor Necrosis Factor: Structure, Function and Mechanism of Action. Marcel Dekker, 1991 Inc., New York

Beutler B., Cerami A. The biology of cachectin/TNF-a primary mediator of the host response. Annu Rev Immunol 1989;7:625-55.

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