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TNF-alpha (pig) ELISA kit

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    TNF-alpha (pig) ELISA kit
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  • TNF-alpha (pig) ELISA kit
Cat No: A05411
Assays - Hrp Kits
SPI-Bio
€390.00
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Known as cachectin, the TNF-alpha (Tumor Necrosis Factor-alpha) is a glycoprotein of 156 amino acids produced from a precursor processed by action of a metalloprotease. Isolated for the first time in 1975 by Carswell, it’s a cytokine of the innate immunity.
Interleukin-1 or bacterial endotoxins stimulate the production of TNF-alpha by immune cells (activated macrophages, NK cells, monocytes) and non-immune cells (keratinocytes, vascular smooth muscle cells, and granulosa lutein cells).
The main function of the TNF-alpha is to act as an immunomodulator and as a mediator of infl ammatory responses in the phenomena of host resistance to infection. It allows neutrophils and macrophages recruitment to infectious sites by stimulating the production of adhesion molecules and chemokines (chemotactic cytokines) by endothelial cells. TNFalpha also acts on the hypothalamus to trigger the fever.
Over-production of TNF-alpha has been implicated in a number of pathological conditions including cachexia, septic shock, and autoimmune disorders.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6 months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,02 ng/mL
Standard Curve Range: 0,02-2,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104

Carswell E. A., Old L. J., Kassel R. L. et al. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA (1975) 72:3666-70

Trebichavský I., Splíchal I., Barot-Ciorbaru R. Expression of TNF-alpha in pig fetal cells stimulated in vitro. Folia Microbiol (Praha).1995;40(4):417-20

Allen D.G., Riviere J.E., Monteiro-Riviere N.A. Cytokine induction as a measure of cutaneous toxicity in primary and immortalized porcine keratinocytes exposed to jet fuels, and their relationship to normal human epidermal keratinocytes. Toxicol Lett. 2001 Mar 8;119(3):209-17

Newman W.H., Zhang L.M., Lee D.H. et al. Release of tumor necrosis factor-alpha from coronary smooth muscle: activation of NF-kappaB and inhibition by elevated cyclic AMP. J Surg Res. 1998 Dec;80(2):129-35

Vézina S. A., Roberge D., Fournier M. et al. Cloning of porcine cytokine-specifi c cDNAs and detection of porcine tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-1 beta gene expression by reverse transcription PCR and chemiluminescence hybridization. Clin Diagn Lab Immunol. 1995 Nov;2(6):665-71

Sundgren-Andersson A.K., Ostlund P., Bartfai T. IL-6 is essential in TNF-alpha-induced fever. Am J Physiol. 1998 Dec;275(6 Pt 2):R2028-34

Vilcek J., Lee T.H. Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions J Biol Chem 1991 Apr 25;266(12):7313-6

Ware C.F. et al Apoptosis mediated by the TNF-related cytokine and receptor families. J. Cellular Biochemistry 1996 60:47

Aggarwal B.B., Vilcek J., eds Tumor Necrosis Factor: Structure, Function and Mechanism of Action. Marcel Dekker, 1991 Inc., New York

Beutler B., Cerami A. The biology of cachectin/TNF-a primary mediator of the host response. Annu Rev Immunol 1989;7:625-55.

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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