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Histamine ELISA kit

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    Histamine ELISA kit
  • Histamine ELISA kit
Cat No: A05890
Assays - Ache Kits
Price is excluding VAT and does not include packaging neither shipping

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

: 96 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Histamine is a natural amine present in many animal and human tissues. It was considered to be the mediator of immediate allergy. Histamine plays a role in various physiological processes, such as control of gastric acid secretion, neurotransmission and modulation of inflammatory and immunological reactions. This competitive enzyme-immunoassay uses a prederivation (derivate reagent with a structure close to the spacer arm used for the immunogen preparation) of histamine that allows to enhance the sensitivity of the assay.
Size: 96 wells
Shipping: Dry ice
Stability: Store at -20degrees; shelf life 1 year maximum after production
Application Media: Plasma, urine, culture supernatant as well as liquid (e.g. broncho-alveolar lavage fluids) or solid (brain, nervous tissus) biological samples|Plasma, urine and culture supernatant: no extraction required|liquid or solid biological samples: extraction HCI
Sample volume: 100 µL
Tracer: AcetylCholinesterase AChE
Detection Limit: 0.5 nM
Standard Curve Range: 0.4-50 nM
Custom Code: 3822000000
UNSPSC code: 41116104


Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34


Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011


Kassas-Guediri A1,2, Coudrat J1,2, Pacreau E et al. Phospholipid scramblase 1 amplifies anaphylactic reactions in vivo. PLoS One. 2017 Mar 10 ;12(3) : e0173815

Shimoda T, Obase Y, Matsuse H et al. The Pathogenesis of Alcohol-Induced Airflow Limitation in Acetaldehyde Dehydrogenase 2-Deficient Mice. Int Arch Allergy Immunol. 2016 ; 171(3-4) :276-284

Choi H, Masaru Tanaka M and Sugimoto K, Difference in Cell Proliferation and Spontaneous Mediator Release Between Two Mast Cell Lines, NCL-2 and RBL-2H3 on Honey Comb-like Structured Film. J Nanotechnol Nanomed Nanobiotechnol 2016, 3 : 009

Isaka M, Befu M, Matsubara N et al. Histamine concentration is involved in canine valvular disease. Veterinary Science Development 2014 ; 4 :5123

Graf A. et al. Knockout of histidine decarboxylase decreases bile duct ligationinduced biliary hyperplasia via downregulation of the histidine decarboxylase/VEGF axis through PKA-ERK1/2 signaling. Am J Physiol Gastrointest Liver Physiol 307: G813–G823, 2014

Saligrama N. et al. Systemic Lack of Canonical Histamine Receptor Signaling Results in Increased Resistance to Autoimmune Encephalomyelitis. J Immunol 2013; 191:614-622

Shiraishi Y. et al. Sequential Engagement of FcƐRI on Mast Cells and Basophil Histamine H4 Receptor and FcƐRI in Allergic Rhinitis. J Immunol 2013; 190:539-548

Kim Y. et al. Histone Deacetylase 3 Mediates Allergic Skin Infl ammation by Regulating Expression of MCP1 Protein*. J. Biol. Chem. 2012, 287:25844-25859

Skovgaard N. et al. Histamine induces postprandial tachycardia through a direct effect on cardiac H2-receptors in pythons. Am J Physiol Regulatory Integrative Comp Physiol 296:774-785, 2009

Yanagisawa R. et al. Titanium Dioxide Nanoparticles Aggravate Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. Exp Biol Med 234:314–322, 2009

Nautiyal K. et al. Brain mast cells link the immune system to anxiety-like behavior. PNAS (2008) 10.1073/pnas.0809479105 Supporting Information

Struthers S. et al. Pharmacological Characterization of a Novel Nonpeptide Antagonist of the Human Gonadotropin-Releasing Hormone Receptor, NBI-42902. Endocrinology 2007 148 :857-867

Noguchi J et al. Strain difference of murine bone marrow-derived mast cell functions. J. Leukoc. Biol. 78 : 605–611 ; 2005

Furutani K et al. Crucial Role of Histamine for Regulation of Gastric Acid Secretion Ascertained by Histidine Decarboxylase-Knockout Mice. JPET 307 :331–338, 2003

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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