Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Size | 96 wells |
Shipping | Dry ice |
Specificity | |
Application Media | Plasma and serum|No extraction required; inhibitor cocktail to prevent hemolysis consequences |
Sample volume | 50 µL |
Tracer | AcetylCholinesterase AChE |
Detection Limit | 0.08 ng/mL |
Standard Curve Range | 0.08-10 ng/mL |
Custom Code | 3822000000 |
UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION: INSULIN
Wilcox G. Insulin and Insulin Resistance. Clin Biochem Rev Vol 26 May 2005
PRODUCT EXPLANATION: KIT DEVELOPMENT
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
CITATIONS OF SPI-BIO INSULIN KIT
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El-Seweidy MM, El-Sayed Asker M, Atteia HH, Hegazy SMS. Atorvastatin and Fenofibrate Modulate Certain Steroidal Hormones, Vitamin D and Bile Acids in Diabetic Dyslipidemic Rats. International Journal of Pharmaceutical and Clinical Research 2015; 7(6): 426-434
Tallino S, Duffy M, Ralle M et al. Nutrigenomics analysis reveals that copper deficiency and dietary sucrose up-regulate inflammation, fibrosis and lipogenic pathways in a mature rat model of nonalcoholic fatty liver disease. J Nutr Biochem. 2015 Oct;26(10):996-1006
Ammar HI, Sequiera GL, Nashed MB et al. Comparison of adipose tissue- and bone marrow- derived mesenchymal stem cells for alleviating doxorubicin-induced cardiac dysfunction in diabetic rats. Stem Cell Res Ther. 2015 Aug 22;6:148
Vella RE, Pillon NJ, Zarrouki B et al. Ozone exposure triggers insulin resistance through muscle c-Jun N-terminal kinase activation. Diabetes. 2015 Mar;64(3):1011-24
Hadji L, Berger E, Soula H et al. White adipose tissue resilience to insulin deprivation and replacement. PLoS One. 2014 Aug 29;9(8):e106214
Wong TW, Sumiran N, Mokhtar MT, Kadir A. Blood glucose lowering property of water in oral insulin-fed diabetic rats. Pharm Biol. 2012 Nov;50(11):1463-6
Ibrahim MA, Ibrahim SA, Amin EF et al. Selective versus non selective cyclooxygenase inhibitors in high fructose-induced metabolic Syndrome. Egyptian Journal of Basic and Clinical Pharmacology, December 2012, Vol. 2, No.2: 37: 34
Baquié M, St-Onge L, Kerr-Conte J et al. The liver receptor homolog-1 (LRH-1) is expressed in human islets and protects b-cells against stress-induced apoptosis. Human Molecular Genetics, 2011, Vol. 20, No. 14 : 2823–2833