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Assays: cell based, immunoassay, LC-MS/MS…

Bertin Bioreagent offers a wide range of Assays based on different technologies: Antibodies, Enzyme/Substrate, Enzyme/Dye Complexes, Fluorescent Detection, Oligonucleotide Cleavage Assay, Hypernated Techniques.

Our wide range of Assays

cell based assays
transcription factors assay kits
enzymatic immunoassays
enzymatic immunoassays for biomarkers
membrane proteine quantification assay kit by LC-MS/MS
DNA repair assay kit

Enzyme ImmunoAssay

Kinetic superiority and high sensibility

AChE® shows true first-order kinetics with a turnover of 64,000 sec-1. That is nearly 3 times faster than HRP or alkaline phosphate. AChE® allows a greater sensitivity than other labelling enzymes.

Low background

Non enzymatic hydrolysis of acetylthiocholine substrate in buffer is essentially absent. So, AChE® allows a very low background and an increased signal/noise ratio compared to other enzyme substrates which are inherently unstable.

Wide dynamic range

AChE® is a stable enzyme and its activity remains constant for many hours as, unlikely to other enzymes, its substrate is not suicidal. This permits simultaneous assays of high diluted and very concentrated samples.

Versatility

AChE® is a completely stable enzyme. Thus, if a plate is accidentally dropped after dispatch of the AChE® substrate (Ellman’s reagent), one only needs to wash the plate, add fresh Ellman’s reagent and proceed with a new development.

Above are the main features of the 4 technologies used in our EIA assays. In order to acquaint you with those techniques, we propose on demand training sessions adapted for either beginners or advanced scientists. Please contact us to learn more about programs and dates.

Assay formats used : Enzyme Linked Immuno Sorbent Assay (EIA, ELISA), Competitive ImmunoAssay, Two-site (sandwich) ImmunoAssay, Solid-Phase Immobilized Epitote Immunometric Assay (SPIE-IA)