Nitro Blue Tetrazolium (chloride

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Nitro Blue Tetrazolium (chloride)

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Cat No: 17341

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative.{22804} It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which...

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Territorial Availability: Available through Bertin Technologies only in France

Synonyms:

2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium, dichloride

Correlated keywords:

7695-60-5 67740-22-1 83800-46-8 87714-63-4 121287-37-4 141102-30-9 562102-25-4 679787-05-4 867000-48-4 1401448-88-1 1422392-93-5 NSC27622 nitro BT TB NTB biochemical dye stain indicator cell biology reactive probe redox-sensitive chromogenic

Product Overview:
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative.{22804} It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease.{28623,28624} NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions.{28625} More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity.{28626} Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Size	250 mg
Shipping	dry ice
CAS Number	298-83-9
Molecular Formula	C₄₀H₃₀N₁₀O₆ • 2Cl
SMILES	COC1=CC(C2=CC(OC)=C([N+]3=NC(C4=CC=CC=C4)=NN3C5=CC=C([N+]([O-])=O)C=C5)C=C2)=CC=C1[N+]6=NC(C7=CC=CC=C7)=NN6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
Molecular Weight	817,6
Formulation	A crystalline solid
Custom Code	2933.19
UNSPSC code	12352100

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.