Coelenterazine 400a

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Coelenterazine 400a

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Cat No: 16157

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET...

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Territorial Availability: Available through Bertin Technologies only in France

Synonyms:

6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one

Correlated keywords:

fluorescent probes intracellular signaling coelenterazine hh bisdeoxycoelenterazine BDC bisdeoxy derivatives Renilla luciferase BRET BRET1 BRET2 proteins protein-protein interactions Rluc GFP receptors luciferin substrates photoproteins oxidation energy dideoxycoelenterazine Deep Blue C DeepBlueC™

Product Overview:
Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.{26296} Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.{26291,26296} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}

Size	500 µg
Shipping	dry ice
CAS Number	70217-82-2
Molecular Formula	C₂₆H₂₁N₃O
SMILES	O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31
Molecular Weight	391,5
Formulation	A crystalline solid
Purity	≥95%
Custom Code	2922.19
UNSPSC code	12352100

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.