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Urea Fluorometric Assay Kit

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    Urea Fluorometric Assay Kit
  • Urea Fluorometric Assay Kit
Cat No: 700620
Assay Kits - Fluorometric Assays
Cayman
€409.00
Price is excluding VAT and does not include packaging neither shipping

Urea or carbamide is synthesized in the body of many organisms as part of the urea cycle. It is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides i...

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: 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • kits assays measures measurements carbamide cycle protein amino acids aa metabolisms catabolism deaminated ammonia livers N-acetylglutamate blood kidneys urine nitrogen countercurrent exchange systems nephrons kidneys creatinine prerenal hperuremia cardiac decompensation water depletion renal glomerulonephritis chronic nephritis polycystic nephrosclerosis tubular necrosis urinary tract toxic metabolites ammonium hyperammonemia plasma detoxification neurological brain neuroprotection serum urease hydrolysis carbon dioxide CO2 detector fluorescence fluorescent
Product Overview:
Urea or carbamide is synthesized in the body of many organisms as part of the urea cycle. It is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays a role in the countercurrent exchange system of the nephrons, allowing for re-absorption of water and critical ions from the excreted urine. Cayman’s Urea Assay provides a convenient method for detecting urea in plasma, serum, and urine. In this assay, urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Ammonia reacts with the detector resulting in a fluorescent product. Fluorescence is analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.
Size 96 wells
Shipping dry ice
Stability Store at -20 degrees; shelf life 365 days
Custom Code 3822.00
UNSPSC code 41116104

Sands, J.M., and Layton, H.E. The physiology of urinary concentration: An update. Semin Nephrol 29(3) 178-195 (2009).

Walker, V. Ammonia toxicity and its prevention in inherited defects of the urea cycle. Diabetes, Obesity and Metabolism 11 823-835 (2009)

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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