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Mouse RORγ Reporter Assay System, 1 x 96-well format assays

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    Mouse RORγ Reporter Assay System, 1 x 96-well format assays
  • Mouse RORγ Reporter Assay System, 1 x 96-well format assays
Cat No: M04001
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€1,050.00
Price is excluding VAT and does not include packaging neither shipping

INDIGO’s Mouse RORγ (RAR-related Orphan Receptor Gamma; nr1f3) referred to as mouse rorC, or here as mRORγ. The N-terminal DNA binding domain (DBD) of the native mRORγ receptor has been substituted with that of the yeast GAL4-DBD. The principle applica...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • M0400x
  • Mouse
  • RORg
  • (mRORG;
  • nr1f3)
Correlated keywords:
  • Cell-Based Assay
  • Endocrinology
  • Gene Regulation
  • Nuclear Receptors
  • Signal Transduction
  • Assay Kits
  • Nuclear Receptors
  • Steroids
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Product Overview:
INDIGO’s Mouse RORγ (RAR-related Orphan Receptor Gamma; nr1f3) referred to as mouse rorC, or here as mRORγ. The N-terminal DNA binding domain (DBD) of the native mRORγ receptor has been substituted with that of the yeast GAL4-DBD. The principle application of this reporter assay is in the screening of test samples to quantify inverse-agonist or agonist activities that they may exert against mouse RORγ.
RAR-related orphan receptor gamma (ROR-gamma), also known as NR1F3 (nuclear receptor subfamily 1, group F, member 3) is a nuclear receptor encoded by the RORG gene.The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors.[1][2] There are three forms of ROR, ROR-α, -β, and -γ and each is encoded by a separate gene (RORA, RORB, and RORC respectively). The RORs are somewhat unusual in that they appear to bind as monomers to hormone response elements as opposed to the majority of other nuclear receptors which bind as dimers. Melatonin has been reported to be the endogenous ligand for ROR-α while CGP 52608 has been identified as a ROR-α selective synthetic ligand. However X-ray crystallographic (PDB 1n83 and 1s0x) and functional data both suggest that cholesterol or a cholesterol derivative may be the endogenous ligand. In contrast, all-trans retinoic acid binds with high affinity to ROR-β and -γ but not ROR-α.
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

mouse (mRORγ)

Specificity
Custom Code 3822000002
UNSPSC code 41116104

http://indigobiosciences.com/ortholog-nr-assays/mouse-rar-related-orphan-receptor-gamma-mrorg-nr1f3/

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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