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Human Nrf2 Reporter Assay System, 1 x 96-well format assay

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    Human Nrf2 Reporter Assay System, 1 x 96-well format assay
  • Human Nrf2 Reporter Assay System, 1 x 96-well format assay
Cat No: IB10001
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€1,050.00
Price is excluding VAT and does not include packaging neither shipping

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a ubiquitously expressed, basic leucine zipper transcription factor. It regulates the expression of a variety of genes,encoding proteins that play critical roles in cyto-protection, as well as the d...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • CAY-17734
  • NFE2L2
  • Nuclear Factor (erythroid-derived 2)-like 2
  • IB1000x
  • Nrf2
  • (non-NR
  • assay)
Correlated keywords:
  • Cell-Based Assay
  • Apoptosis
  • Cell Biology
  • Assay Kits
  • Fluorescent Probes
  • apoptosis
  • assay
  • kit
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Product Overview:
Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a ubiquitously expressed, basic leucine zipper transcription factor. It regulates the expression of a variety of genes,encoding proteins that play critical roles in cyto-protection, as well as the detoxification and clearance of harmful endogenous and xenobiotic substances. In particular, Nrf2 regulates the expression of antioxidant proteins that confer cyto-protection against oxidative damage. Under normal conditions Nrf2 resides in the cytoplasm in association with Keap1 and Cullin 3. Within the confines of this protein cluster Nrf2 is the target of ubiquitination and rapid turn-over via proteasomal degradation. However, under conditions of cellular oxidative stress the tight association of Nrf2 with Keap1 and Cullin 3 is broken, effectively disrupting the otherwise efficient process of Nrf2 degradation. Once non-ubiquitinated Nrf2 accumulates in the cytoplasm it translocates into the nucleus, whereupon it forms hetero-dimers with Maf. In this configuration Nrf2 binds to antioxidant response element (ARE) sequences resident in the promoter regions of some genes, initiating transcription complex formation, and culminating in the expression of antioxidant proteins.
Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a ubiquitously expressed, basic leucine zipper transcription factor. It regulates the expression of a variety of genes encoding proteins that play critical roles in cyto-protection, as well as the detoxification and clearance of harmful endogenous and xenobiotic substances. In particular, Nrf2 regulates the expression of antioxidant proteins that confer cyto-protection against oxidative damage. Under normal conditions Nrf2 resides in the cytoplasm in association with Keap1 and Cullin 3. Within the confines of this protein cluster Nrf2 is the target of ubiquitination and rapid turn-over via proteasomal degradation. However, under conditions of cellular oxidative stress the tight association of Nrf2 with Keap1 and Cullin 3 is broken, effectively disrupting the otherwise efficient process of Nrf2 degradation. Once non-ubiquitinated Nrf2 accumulates in the cytoplasm it translocates into the nucleus, whereupon it forms hetero-dimers with Maf. In this configuration Nrf2 binds to antioxidant response element (ARE) sequences resident in the promoter regions of some genes, initiating transcription complex formation, and culminating in the expression of antioxidant proteins.
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

human

Specificity
Custom Code 3822000002
UNSPSC code 41116104

http://indigobiosciences.com/indigo-kits-services/nrf2-assay/

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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