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Human NF-kB Reporter Assay System, 1 x 384-well format assay

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    Human NF-kB Reporter Assay System, 1 x 384-well format assay
  • Human NF-kB Reporter Assay System, 1 x 384-well format assay
Cat No: IB09002
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€2,790.00
Price is excluding VAT and does not include packaging neither shipping

This assay system utilizes HEK293t cells that express NF-kB (nuclear factor kappa-light-chain enhancer of activated B Cells), and contain the luciferase reporter gene functionally linked to upstream NF-kB genetic response elements. Thus, quantifying ch...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • IB0900x
  • NF-Kb
  • (non-NR
  • assay)
Correlated keywords:
  • Cell-Based Assay
  • Endocrinology
  • Gene Regulation
  • Nuclear Receptors
  • Signal Transduction
  • Assay Kits
  • Nuclear Receptors
  • Steroids
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Product Overview:
This assay system utilizes HEK293t cells that express NF-kB (nuclear factor kappa-light-chain enhancer of activated B Cells), and contain the luciferase reporter gene functionally linked to upstream NF-kB genetic response elements. Thus, quantifying changes in luciferase expression provides a sensitive surrogate measure of changes in the level of NF-kB activation.
NF-kB is a signal transduction dependent transcription factor. This NF-kB reporter cell line is validated to provide a robust dose-dependent activation response when treated with TNFa, or the Protein Kinase C activator Phorbol 12-myristate 13-acetate (PMA). As such, the principle application of this assay system is in the screening of test samples to quantify any functional activities that they may exert to modulate, either induce or suppress, NF-kB activities.
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

human

Specificity
Custom Code 3822000002
UNSPSC code 41116104

http://indigobiosciences.com/indigo-kits-services/nf-kb-assay/

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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