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Human ERRγ Reporter Assay System, 1 x 96-well format assay

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    Human ERRγ Reporter Assay System, 1 x 96-well format assay
  • Human ERRγ Reporter Assay System, 1 x 96-well format assay
Cat No: IB08021
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€1,050.00
Price is excluding VAT and does not include packaging neither shipping

This nuclear receptor assay system uses specific non-human mammalian cells modified to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor γ (NR3B3). The N-terminal DNA binding domains (DBD) of the native ERRγ has been...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • CAY-16524
  • Estrogen-Related Receptor γ (human) Reporter Assay Kit
  • NR3B3
  • IB0802x
  • ERRg
  • (ERRG;
  • NR3B3)
Correlated keywords:
  • Cell-Based Assay
  • Circadian Clock
  • Gene Regulation
  • Metabolism
  • Nuclear Receptors
  • Signal Transduction
  • Assay Kits
  • Nuclear Receptors
Show more...
Product Overview:
This nuclear receptor assay system uses specific non-human mammalian cells modified to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor γ (NR3B3). The N-terminal DNA binding domains (DBD) of the native ERRγ has been substituted with that of the yeast GAL4-DBD. The reporter gene is beetle luciferase functionally linked to the GAL4 upstream activation sequence (UAS). As is true in vivo, these reporter cells express ERRγ that is constitutively active in the (putative) absence of a ligand binding event. Interestingly, the ligand binding domain of ERRγ may be occupied by a ligand that further elevates the constitutive activity of the receptor (an agonist response), or one that results in a dose-dependent loss of constitutive active (an inverse-agonist response). Figure 2 demonstrates the high constitutive activity of ERRγ in the absence of added ligand, as well as agonist and inverse-agonist responses of the receptor upon ligand binding. Therefore, the principle application of this assay system is in the screening of test samples to quantify either agonist or inverse-agonist activities that they may exert against human ERRγ. ERRγ Reporter Cells are prepared using Indigo's specific CryoMiteTM process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the preincubation of reporter cells prior to assay setup. Indigo Bioscience's Nuclear Receptor Reporter Assays are all-inclusive cell-based assay systems. In addition to ERRγ Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. IB08021
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

human

Specificity
Custom Code 3822000002
UNSPSC code 41116104

http://indigobiosciences.com/indigo-kits-services/estrogen-related-receptor-gamma-errg-nr3b3/

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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