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Human RORγ Reporter Assay System, 3 x 32 assays in 96-well format

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    Human RORγ Reporter Assay System, 3 x 32 assays in 96-well format
  • Human RORγ Reporter Assay System, 3 x 32 assays in 96-well format
Cat No: IB04001-32
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€1,117.00
Price is excluding VAT and does not include packaging neither shipping

This human RAR-related orphan receptor gamma (RORγ) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORγ...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • CAY-15757
  • IB0400x
  • RORg
  • (RORG;
  • NR1F3)
Correlated keywords:
  • Cell-Based Assay
  • Bone-Related Research
  • Cancer
  • Gene Regulation
  • Intracellular Signaling
  • Nuclear Receptors
  • Signal Transduction
  • Assay Kits
Show more...
Product Overview:
This human RAR-related orphan receptor gamma (RORγ) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORγ. Variant 2 mRNA comprises an alternate exon 1 that replaces the exon 1 and 2 sequences found in the Variant 1 transcript. Consequently, variant 2 mRNA presents a unique 5'UTR and modified N-terminal ORF sequences, resulting in the expression of a shorter isoform 2 receptor. The isoform 2 receptor is expressed predominantly in specialized immune cells developing within the thymus; as such it is referred to as RORγt. This nuclear receptor assay system uses specific human cells modified to provide high-level expression of a hybrid form of the human RAR-related orphan receptor gamma. The N-terminal DNA binding domains (DBD) of the native RORγ and RORγt receptors have been substituted with that of the yeast GAL4-DBD. Hence, the GAL4- RORγ hybrid receptor expressed in these reporter cells will not discern any functional differences that may exist between the native isoform 1 and isoform 2 receptors. As is true in vivo, uncharacterized molecular activators present in these reporter cells maintain RORγ in a constant state of high-level activity. The principal application of this reporter assay system is in the screening of test samples to quantify inverse-agonist activities that they may exert against human RORγ. Reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryopreservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. IB0400
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

human

Specificity
Custom Code 3822000002
UNSPSC code 41116104

http://indigobiosciences.com/indigo-kits-services/rar-related-orphan-receptor-gamma-rorg-nr1f3/

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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