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Human GR Reporter Assay System, 3 x 32 assays in 96-well format

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    Human GR Reporter Assay System, 3 x 32 assays in 96-well format
  • Human GR Reporter Assay System, 3 x 32 assays in 96-well format
Cat No: IB00201-32
Assay Kits - Nuclear Receptor Reporter Assays
Indigo
€1,117.00
Price is excluding VAT and does not include packaging neither shipping

This nuclear receptor assay system uses specific non-human cells modified to provide constitutive high-level expression of full-length, unmodified human glucocorticoid receptor (NR3C1), a ligand-dependent transcription factor, commonly referred to as G...

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: 1 ea
- +
Territorial Availability: Available through Bertin only in Europe
Special Advice: Ship on dry ice to prevent it from thawing.
Synonyms:
  • CAY-15733
  • IB0020x
  • GR
  • (NR3C1)
Correlated keywords:
  • Cell-Based Assay
  • Endocrinology
  • Gene Regulation
  • Inflammation
  • Nuclear Receptors
  • Oxidative Injury
  • Signal Transduction
  • Assay Kits
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Product Overview:
This nuclear receptor assay system uses specific non-human cells modified to provide constitutive high-level expression of full-length, unmodified human glucocorticoid receptor (NR3C1), a ligand-dependent transcription factor, commonly referred to as GR. Indigo’s reporter cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in GR activity. Luciferase gene expression occurs after ligand-bound GR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from Indigo’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. GR reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryopreservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. Indigo Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to GR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. IB0020
Size 1 ea
Shipping dry ice
Stability Store at -80 degrees; shelf life 180 days maximum after production
Application(s)

human

Specificity
Custom Code 3822000002
UNSPSC code 41116104

INDIGO Biosciences, Inc., based in State College, PA, has the largest global portfolio of nuclear receptor (NR) assay kits and related screening services, each with uncompromising robustness and accuracy.

INDIGO was formed with the mission of collapsing the time and cost associated with drug and chemical evaluations. The goal was to identify time-consuming and costly failures before a candidate drug enters the FDA process. By enabling a fast-fail approach in the earliest, pre-clinical phase of drug development, INDIGO’s products and services can save a biopharmaceutical company hundreds of millions of dollars and years of time that are often attributable to potential poor on-target performance and unanticipated off-target behaviors.

INDIGO Biosciences’ nuclear receptor assay products are cell-based reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.

Our reporter systems utilize fire fly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least I hour, allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read-time of assay plates.

INDIGO reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (3x 32-, 1x96-, and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.

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