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Glyco-SPOT DNA Repair Assay kit

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    Glyco-SPOT DNA Repair Assay Kit
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    Glyco-SPOT DNA Repair Assay kit
  • Glyco-SPOT DNA Repair Assay Kit
  • Glyco-SPOT DNA Repair Assay kit
Cat No: LX05001
Bertin Bioreagent
Price is excluding VAT and does not include packaging neither shipping

Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases...

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: 24 wells

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Territorial Availability: Available worldwide directly through Bertin Pharma or your local distributor
Synonyms:
  • DNA repair
  • Base excision Repair
  • BER
  • NER
  • DNA lesion
  • Lesion DNA adducts
Correlated keywords:
  • BER
  • multiplexed
  • DNA
  • Repair
  • Base
  • Excision
  • Repair
  • lesion
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Product Overview:
Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases followed by the processing of the resulting abasic site either by the AP-lyase activity of the bifunctional glycosylase or by the apurinic/apyrimidic endonuclease APE1 that incises the DNA strand. BER mechanisms can be induced by oxidative stress and various genotoxic attacks.
The Glyco-SPOT DNA Repair Assay is a multiplexed oligonucleotide (ODN) cleavage assay developed on support. It is used to monitor simultaneously the cleavage efficiency of several glycosylases/AP endonucleases against a set of emblematic DNA lesions in cell/bacteria/tissue extracts, using fluorescent detection.
The assay can be used to screen for DNA repair inhibitors, characterize DNA repair enzymatic signature from samples, check DNA glycosylase specificity, characterize DNA repair inhibitory properties of chemicals (heavy metals …).
Size 24 wells
Shipping wet ice
Specificity
Custom Code 38220000
UNSPSC code 41116104

Garreau-Balandier I, et al. A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray. FEBS Lett. 2014; 588:1673-9.

Candéias S, et al. Direct inhibition of excision/synthesis DNA repair activities by cadmium: analysis on dedicated Biochips. Mutat Res. 2010; 10:53-9

Pons B, et al. Age-associated modifi cations of Base Excision Repair activities in human skin fi broblast extracts. Mech Ageing Dev. 2010;131:661-5

Guerniou V, et al. Repair of oxidative damage of thymine by HeLa whole-cell extracts: simultaneous analysis using a microsupport and comparison with traditional PAGE analysis. Biochimie. 2005; 87:151-9.

Sauvaigo S, et al. An oligonucleotide microarray for the monitoring of repair enzyme activity toward different DNA base damage. Anal Biochem. 2004; 333:182-92

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