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Anti-Ovalbumin IgG1 (mouse) ELISA Kit

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    Anti-<wbr/>Ovalbumin IgG1 (mouse) ELISA Kit
  • Anti-<wbr/>Ovalbumin IgG1 (mouse) ELISA Kit
Cat No: 500830
Assay Kits - Elisa
Price is excluding VAT and does not include packaging neither shipping

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response.{20137,20136,20138,20141,20140,20139} OVA/alum immunize...

: 96 wells

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Territorial Availability: Available through Bertin Technologies only in Europe
Correlated keywords:
  • EIAs enzymes immunoassays immunometric kits assays measures measurements eggs whites immunization mice mouse chickens albumin OVA precipitate complexes aluminum hydroxides alum TH2-mediated immune response immunity OVA/alum immunized anti-OVA antibodies IgG1 IgE isotypes mediate tissue-specific tissues effectors chronic inflammation allergic asthma rhinitis cutaneous hypersensitivity antibody plasma serum immunoglobulin OVA-specific TH2 antiova ova
Product Overview:
Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response.{20137,20136,20138,20141,20140,20139} OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic inflammation, including allergic asthma, allergic rhinitis, and cutaneous hypersensitivity.{20137,20136,20138,20141} When using one of these models, it is often desirable to measure anti-OVA antibody levels in the plasma or serum to determine the effectiveness of the immunization, the activity of a drug, or the effect of a specific gene deletion. IgG1 is the predominant anti-OVA immunoglobulin isotype found in the serum or plasma of mice immunized with OVA/alum; the plasma concentration of OVA-specific IgG1 is typically 1,000-fold greater than that of OVA-specific IgE.{20137} Therefore, the measurement of anti-OVA IgG1 is a commonly used method of assessing the magnitude of this TH2 immune response. Cayman’s Anti-Ovalbumin IgG1 (mouse) ELISA Kit is an immunometric (i.e., “sandwich”) assay which can be used to measure anti-ovalbumin of the IgG1 isotype in mouse plasma and serum without prior sample purification. Affinity-purified anti-ovalbumin IgG1 isolated from the plasma of mice immunized with OVA/alum is used as the standard. The standard curve spans the range of 1.56-200 ng/ml, with an LLOQ of 1.56 ng/ml.
Size 96 wells
Shipping wet ice
Custom Code 3822.00
UNSPSC code 41116104

Kung, T.T., Jones, H., Adams, G.K.I., et al. Characterization of a murine model of allergic pulmonary inflammation. Int Arch Allergy Immunol 105(1) 83-90 (1994).

Pichavant, M., Goya, S., Hamelmann, E., et al. Animal models of airway sensitization. Curr Pr

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