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LSD1 Inhibitor Screening Assay Kit

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    LSD1 Inhibitor Screening Assay Kit
  • LSD1 Inhibitor Screening Assay Kit
Cat No: 700120
Assay Kits - Fluorometric Assays
Price is excluding VAT and does not include packaging neither shipping

Lysine-specific demethylase 1 (LSD1) is a histone demethylase whose actions on specific lysine residues repress transcription of chromosomal DNA. LSD1 also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue. Inhib...

: 96 wells

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Correlated keywords:
  • lysines 4 demethylases histones residues repression transcription chromosomal DNA chromosomes inhibits inhibitors inhibition tumors suppressors activity cells cycles progression cancers fluorescent fluorescence enzymes enzymatic reactions H2O2 hydrogens peroxides peptides N-terminal tails H3 horseradish peroxidases ADHP resorufins assays
Product Overview:
Lysine-specific demethylase 1 (LSD1) is a histone demethylase whose actions on specific lysine residues repress transcription of chromosomal DNA. LSD1 also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue. Inhibitors of LSD1 are important tools used to elucidate mechanisms of transcription and cell cycle progression and have therapeutic potential for treating cancer. Cayman’s LSD1 Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening LSD1-specific inhibitors. The assay is based on the multistep enzymatic reaction in which LSD1 first produces H2O2 during the demethylation of lysine 4 on a peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3. In the presence of horseradish peroxidase, H2O2 reacts with ADHP to produce the highly fluorescent compound resorufin that can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Size 96 wells
Shipping dry ice
Stability Store at -80 degrees; shelf life 365 days
Custom Code 3822.00
UNSPSC code 41116104

Schmidt, D.M.Z., and McCafferty, D.G. trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1. Biochemistry 46 4408-4416 (2007).

Scoumanne, A., and Chen, X. Protein methylation: A new mechanism of

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Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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