ROCK1 (human, recombinant)

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Cat No: 32757

Proteins - Enzymes

Rho-associated protein kinase 1 (ROCK1) is a serine/threonine kinase that has roles in cytoskeletal organization, as well as cell survival, proliferation, and apoptosis.{60550,60551} It exists as a homodimer where each monomer is composed of an N-term...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

mypt MLC-2

Product Overview:
Rho-associated protein kinase 1 (ROCK1) is a serine/threonine kinase that has roles in cytoskeletal organization, as well as cell survival, proliferation, and apoptosis.{60550,60551} It exists as a homodimer where each monomer is composed of an N-terminal kinase domain, a coiled-coil region that contains the Rho-binding domain, and a C-terminal pleckstrin homology (PH) domain that contains a cysteine-rich C1 domain and acts as an autoinhibitory region.{60552,60551} Rock1 mRNA is expressed in all tissues except in the muscle and brain, and the protein is localized predominantly in the cytosol but also associates with the plasma membrane and centrosomes.{60551,60550} Binding of ROCK1 to GTP-bound RhoA induces activation of ROCK1, which phosphorylates a variety of downstream targets, including myosin light chain (MLC) phosphatase 1 (MYPT1) and MLC2, that have diverse roles in cytoskeletal organization.{60551} The C-terminal autoinhibitory region of ROCK1 is cleaved by caspase-3 during the execution phase of apoptosis, resulting in constitutively active ROCK1 and plasma membrane blebbing, a hallmark of apoptosis.{60550,60551} ROCK1 is overexpressed in tumors from patients with various solid tumors, including hepatocellular carcinoma, glioblastoma, or melanoma, and increased tumor ROCK1 mRNA transcript levels are correlated with increased metastasis and reduced overall survival in patients with breast cancer.{60553,60554} Cayman’s ROCK1 (human, recombinant) protein can be used for ELISA, enzyme activity assay, and Western blot (WB) applications.

Size	10 µg
Shipping	dry ice
Formulation	20 mM HEPES, pH 7.5, with 150 mM sodium chloride, and 1 mM DTT
Purity	≥90% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.