ALK5 Extracellular Domain (human

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ALK5 Extracellular Domain (human, recombinant)

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Cat No: 31847

Proteins - Enzymes

Activin receptor-like kinase 5 (ALK5), also known as TGF-β receptor type 1 (TGF-βRI), is a type I transmembrane glycoprotein, serine/threonine kinase, and member of the TGF-β superfamily that is ubiquitously expressed.{54901,60413} It is composed of a...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

TGF? TGF?RI TGF?RII TGF?-RI SMAD GDF11 TGFBR TGFBR1 TGFR TGFR1 Activin-like Kinase 5 ALK-5

Product Overview:
Activin receptor-like kinase 5 (ALK5), also known as TGF-β receptor type 1 (TGF-βRI), is a type I transmembrane glycoprotein, serine/threonine kinase, and member of the TGF-β superfamily that is ubiquitously expressed.{54901,60413} It is composed of an extracellular ligand-binding domain, a single transmembrane domain, an intracellular serine/threonine kinase domain, and a cytoplasmic serine/threonine-rich region.{54901} The ALK5 ligand TGF-β mediates the formation of a complex that includes ALK5 and the type II transmembrane glycoprotein TGF-βRII, which phosphorylates ALK5 to induce intracellular signaling and phosphorylation of SMAD2 and SMAD3 to regulate gene expression.{54901,60414} The ligands myostatin and growth differentiation factor 11 (GDF-11) induce formation of a complex between ALK5, TGF-βRI, activin receptor type 2A (ACTRIIA), and ACTRIIB to activate SMAD signaling in a similar manner. ALK5 signaling has roles in endothelial cell proliferation and migration, as well as angiogenesis.{60415,60413} Mutations in TGFBR1 are associated with Loeys-Dietz syndrome, which is a connective tissue disorder characterized by a variety of mild-to-severe vascular defects, including aortic aneurysm.{60417} Cayman’s ALK5 Extracellular Domain (human, recombinant) protein can be used for binding assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of ALK5 (amino acids 34-125) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 340 amino acids, has a calculated molecular weight of 38.1 kDa, and a predicted N-terminus of Leu34 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 45-50 kDa by SDS-PAGE under reducing conditions.

Size	100 µg
Shipping	dry ice
Formulation	Lyophilized from sterile PBS, pH 7.4
Purity	≥85% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.