PKM2 (human, recombinant)

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Cat No: 32564

Proteins - Enzymes

Pyruvate kinase (PK) is an enzyme that catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP during aerobic glycolysis.{57358} PKM2 is an isoform of PK encoded by PKM in humans that is primarily expressed during embryonic develop...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

OIP THBP Pyruvate kinase BcrAbl ARAF FGFR STAT NF?B HIF1? Escherichia

Product Overview:
Pyruvate kinase (PK) is an enzyme that catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP during aerobic glycolysis.{57358} PKM2 is an isoform of PK encoded by PKM in humans that is primarily expressed during embryonic development and localized to the cytosol. PKM2 expression is attenuated in adult tissues, however, it is reactivated in cancer cells during tumor development.{57358,57359,57361} It functions as a tetramer in the cytosol that is formed or dissociated in response to post-translational modifications and various allosteric regulators, such as fructose-1,6-bisphosphate, as well as the oxidation of its cysteine residues.{57360} It interacts with various tyrosine kinases, such as Bcr-Abl, A-RAF, and FGFR1, in a reciprocal interaction that regulates PKM2 enzyme kinetics and kinase signaling.{57359} PKM2 also functions as a dimer that can translocate to the nucleus and activate various transcription factors, including STAT3, NF-κB, and HIF-1α, to induce gene transcription.{57361,57359} Levels of PKM2 are increased in mesothelioma, osteosarcoma, and hepatomas, as well as ovarian, breast, gallbladder, and gastric cancers, and are associated with poor prognosis. Cayman’s PKM2 (human, recombinant) protein can be used for enzyme assay applications.

Size	20 µg
Shipping	dry ice
Formulation	40 mM Tris-HCl, pH 8.0, with 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT
Purity	≥90% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.