AMPK Complex (?1, ?1, and ?1 sub

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AMPK Complex (?1, ?1, and ?1 subunits; human, recombinant)

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Cat No: 32557

Proteins - Enzymes

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase.{57355} It is composed of an α subunit, two β subunits, and two γ subunits. The α and β subunits each have two isoforms, designated as α1, α2, β1, and β2, which differ at ...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

AA MGC 33776 57364 PRKAA 17785 PRKAB 8666 PRKAG 1 2 3 FA

Product Overview:
AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase.{57355} It is composed of an α subunit, two β subunits, and two γ subunits. The α and β subunits each have two isoforms, designated as α1, α2, β1, and β2, which differ at residues affected by post-translational modifications, and the γ subunit has γ1, γ2, and γ3 isoforms. The diversity of isoforms that can comprise the AMPK complex impart differing functions and subcellular localization.{57355,57356} AMPK is ubiquitously expressed in eukaryotes, and AMPK complexes containing an α1 subunit are localized to the cytoplasm.{57356} AMPK is activated by phosphorylation of the α subunit by kinases and/or by AMP or ADP binding to the γ subunit, which occurs under low energy conditions with high ratios of AMP/ADP to ATP, or an increase in intracellular calcium levels.{57355,57357} In this way, AMPK acts as a regulator of cellular energy homeostasis. It can also be activated under conditions of oxidative stress, where it functions as a redox sensor.{57356} Its activity is decreased under high energy conditions where ATP, glucose, fatty acids, and amino acids are plentiful.{57355} AMPK is involved via its downstream signaling pathways in lipid and glucose metabolism, protein synthesis, autophagy, and redox regulation, as well as inhibition of inflammatory processes.{57355,57357} AMPK activation in vivo reduces adiposity in mice, as well as inhibits proliferation of cancer cells in vitro and reduces tumor growth in mouse xenograft models.{57356} Cayman's AMPK α1, β1, and γ1 Subunits (human, recombinant) protein can be used for enzyme activity assay applications. This protein consists of 1,160 amino acids and has a calculated molecular mass of approximately 146 kDa.

Size	10 µg
Shipping	dry ice
Formulation	50 mM Sodium phosphate, pH 7.0, with 300 mM sodium chloride, 150 mM imidazole, 0.25 mM DTT, and 25% glycerol
Purity	≥90% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

Technical File

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.