B7-1/CD80 Long Isoform Extracell

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B7-1/CD80 Long Isoform Extracellular Domain (human, recombinant; His-tagged)

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Cat No: 32013

Proteins - More Proteins

CD80, also known as B7-1, is a glycoprotein and member of the CD28/B7 family of co-stimulatory receptors that promotes T cell activation.{46976,46983} Alternative splicing of CD80 produces one full-length long isoform, CD80 long, and two short isoform...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

B7.1 BB1 CD28LG CD28LG1 LAB7 CD8 CD-80 28 costimulatory extra-cellular Ig V CTLA4 s1 s2 proinflammatory antiinflammatory IL10 interleukin SDSPAGE

Product Overview:
CD80, also known as B7-1, is a glycoprotein and member of the CD28/B7 family of co-stimulatory receptors that promotes T cell activation.{46976,46983} Alternative splicing of CD80 produces one full-length long isoform, CD80 long, and two short isoform, s1CD80 and s2CD80.{46983} CD80 long exists as a membrane-bound dimer and is composed of an extracellular immunoglobulin variable (IgV) domain that interacts with the co-stimulatory molecule CD28 (Item No. 32014) or the inhibitory molecule CTLA-4 (Item No. 32009), as well as an immunoglobulin constant (IgC) domain and a cytoplasmic tail that are both required for T cell co-stimulation.{46976} s1CD80 and s2CD80 lack the transmembrane domain or the transmembrane domain and the IgC domain, respectively, and are found in the serum.{46983} CD80 is transiently expressed on the surface of antigen-presenting cells (APCs) and is upregulated following ligation of the co-stimulatory molecule CD86, as well as by pro-inflammatory stimuli, such as LPS, and downregulated by the anti-inflammatory cytokine IL-10.{55164,55165,55166} CD80 has two ligands, CD28 and CTLA-4, that compete for binding and are each expressed on naïve T cells but have opposing functions on T cell activation.{46977} CD80 binding to CD28 promotes T cell activation, survival, and cytokine production, whereas CD80 binding to CTLA-4 inhibits T cell activation and promotes T cell anergy. Neutralization of CD80 with a monoclonal antibody reduces the severity of synovitis and bone erosion, as well as CD4+ T cell infiltration, in the inflamed joints of a mouse model of arthritis induced by complete Freund's adjuvant (CFA) and BSA.{55167} Decreased CD80 tumor levels have been identified in patients with renal cell carcinoma.{55168} CD80 SNPs have been found in individuals with gastric cancer.{55169} Cayman's CD80 Long Isoform Extracellular Domain (human, recombinant) protein can be used for binding assay applications. This protein consists of 219 amino acids, has a calculated molecular weight of 25.4 kDa, and a predicted N-terminus of Val35 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of Cayman's CD80 Long Isoform Extracellular Domain (human, recombinant) protein is approximately 45 to 48 kDa due to glycosylation.

Size	100 µg
Shipping	dry ice
Formulation	Lyophilized from sterile PBS, pH 7.4
Purity	≥95% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352202

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.