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S-Glutathionylated Protein Detection Kit

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    S-<wbr/>Glutathionylated Protein Detection Kit
  • S-<wbr/>Glutathionylated Protein Detection Kit
Cat No: 10010721
Cayman

Glutathione (GSH) is a tripeptide (γ-glutamylcysteinylglycine) widely distributed in both plants and animals.{1813,4672} GSH is involved in maintenance of protein sulfhydryl reduction status.{4669,4670} The concentration of GSH ranges from a few micro...

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Territorial Availability: Available through Bertin Technologies only in France
Correlated keywords:
  • assays kits measures measurements flow cytometry cytometer fluorescence microscopy GSH tripeptides gamma-glutamyl g-glutamyl .gamma.-glutamyl ?-glutamylcsteinylglyceines proteins sufhydryls reduction plasma tissues livers disulfides cells metabolic structural cytoskeletal signaling adduct antibodies Biotin-Switch method whole permeabilized avidin overlay analysis free-thiols enzymatic cleavages protein-S-glutathiones streptavidin-based glutathione
Product Overview:
Glutathione (GSH) is a tripeptide (γ-glutamylcysteinylglycine) widely distributed in both plants and animals.{1813,4672} GSH is involved in maintenance of protein sulfhydryl reduction status.{4669,4670} The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver.{4671,4673} Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest.{11632,17080,15065} Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins.{16795} PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the “Biotin-Switch” method.{15065,13441,12802} Cayman’s S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis.{15066} This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.
Size 1 ea
Shipping dry ice
Custom Code 3822.19
UNSPSC code 41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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