Lipid Droplets Fluorescence Assa

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Lipid Droplets Fluorescence Assay Kit

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Cat No: 500001

Assay Kits - Cell-Based Assays

Cayman’s Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets.{17124} Furt...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

lipids droplets storage organelles cytoplasms eukaryotic cells mammalian adipocytes steroidogenic fatty acids metabolism membranes synthesis phospholipids cholesterols steroids PAT proteins Perilipin adipophilin tail-interacting TIP 47 intracellular homeostasis trafficking signaling immunopathological inflammation anti-inflammatory mediators leukocytes therapy assays kits measures measurements fluorescences fluorescent biogenesis Nile Red microscopy flow cytometry plates readers adipogenesis NASH non-alcoholic nonalcoholic steatohepatitis NAFLD nonalcoholic fatty liver disease

Product Overview:
Cayman’s Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets.{17124} Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescent plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with antibodies conjugated to compatible fluorophores. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagents to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.

Size	480 tests
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.