MAP1LC3B/LC3B (human recombinant

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MAP1LC3B/LC3B (human recombinant)

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Cat No: 25829

Proteins - More Proteins

Microtubule-associated protein 1 light chain 3β (MAP1LC3B), also known as LC3B, is an ortholog of yeast Atg8 that is essential to autophagosome biogenesis.{48159} LC3B is irreversibly cleaved by the cysteine protease ATG4B (Item No. 25380) to its acti...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

atg-8 8F 4B 12 5 16 LC3BI LC3B1 ATG12ATG5ATG16 LC3BII LC-3B MAP 1A 1B LC3 B ATG8F MAP1ALC3 MAP1LC3B-A MAP1LC3BA 1BLC3 Escherichia MAP1A MAP1B

Product Overview:
Microtubule-associated protein 1 light chain 3β (MAP1LC3B), also known as LC3B, is an ortholog of yeast Atg8 that is essential to autophagosome biogenesis.{48159} LC3B is irreversibly cleaved by the cysteine protease ATG4B (Item No. 25380) to its active form LC3B-I, which contains an exposed C-terminal glycine residue needed for autophagosome formation.{42341,42342} LC3B-I is then conjugated to phosphatidylethanolamine in canonical autophagy by the ATG12-ATG5-ATG16 complex to form LC3B-II, which localizes to the autophagosomal membrane.{48159, 47096,47097,47098} Knockdown of LC3B increases the number of ATG5- and ATG16-positive puncta in HeLa cells, indicating a defect in the maturation of autophagosomes.{48159} LC3B expression is upregulated in various cancers and has disease-specific implications on progression and prognosis.{48160,48161} In metastatic and castration-resistant human prostate cancer tumor samples, LC3B expression is upregulated and is positively correlated with reduced disease-specific mortality.{48160} Expression of LC3B is also increased in oral squamous cell carcinomas and is associated with aggressive pathological features and poor prognosis.{48161}

Size	100 µg
Shipping	dry ice
Formulation	50 mM HEPES, pH 8.0, with 150 mM sodium chloride, and 10% glycerol
Purity	≥95% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.