AChE® shows true first-order kinetics with a turnover of 64,000 sec-1. That is nearly 3 times faster than HRP or alkaline phosphate. AChE® allows a greater sensitivity than other labelling enzymes.
Non enzymatic hydrolysis of acetylthiocholine substrate in buffer is essentially absent. So, AChE® allows a very low background and an increased signal/noise ratio compared to other enzyme substrates which are inherently unstable.
AChE® is a stable enzyme and its activity remains constant for many hours as, unlikely to other enzymes, its substrate is not suicidal. This permits simultaneous assays of high diluted and very concentrated samples.
AChE® is a completely stable enzyme. Thus, if a plate is accidentally dropped after dispatch of the AChE® substrate (Ellman’s reagent), one only needs to wash the plate, add fresh Ellman’s reagent and proceed with a new development.
Above are the main features of the 4 technologies used in our EIA assays. In order to acquaint you with those techniques, we propose on demand training sessions adapted for either beginners or advanced scientists. Please contact us to learn more about programs and dates.
Assay formats used : Enzyme Linked Immuno Sorbent Assay (EIA, ELISA), Competitive ImmunoAssay, Two-site (sandwich) ImmunoAssay, Solid-Phase Immobilized Epitote Immunometric Assay (SPIE-IA)
Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases followed by the processing of the resulting abasic site either by the AP-lyase activity of the bifunctional glycosylase or by the apurinic/apyrimidic endonuclease APE1 that incises the DNA strand.
BER mechanisms can be induced by oxidative stress and various genotoxic attacks.
The Glyco-SPOT DNA Repair Assay is a multiplexed oligonucleotide (ODN) cleavage assay developed on support. It is used to monitor simultaneously the cleavage efficiency of several glycosylases/AP endonucleases against a set of emblematic DNA lesions in cell/bacteria/tissue extracts, using fluorescent detection.
The assay can be used to screen for DNA repair inhibitors, characterize DNA repair enzymatic signature from samples, check DNA glycosylase specificity, characterize DNA repair inhibitory properties of chemicals (heavy metals …).
LXRepair can run your DNA Repair assays on any biological matrices (cells, tumors, biopsies, organs, bacteria) whatever your application fields Oncology, Toxicology, Cosmetic and ageing sciences.
LXRepair provides the consistent data as well as detailed reports summarizing the results.
Interested? Send your request directly to firstname.lastname@example.org
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