AChE® shows true first-order kinetics with a turnover of 64,000 sec-1. That is nearly 3 times faster than HRP or alkaline phosphate. AChE® allows a greater sensitivity than other labelling enzymes.
Non enzymatic hydrolysis of acetylthiocholine substrate in buffer is essentially absent. So, AChE® allows a very low background and an increased signal/noise ratio compared to other enzyme substrates which are inherently unstable.
AChE® is a stable enzyme and its activity remains constant for many hours as, unlikely to other enzymes, its substrate is not suicidal. This permits simultaneous assays of high diluted and very concentrated samples.
AChE® is a completely stable enzyme. Thus, if a plate is accidentally dropped after dispatch of the AChE® substrate (Ellman’s reagent), one only needs to wash the plate, add fresh Ellman’s reagent and proceed with a new development.
Above are the main features of the 4 technologies used in our EIA assays. In order to acquaint you with those techniques, we propose on demand training sessions adapted for either beginners or advanced scientists. Please contact us to learn more about programs and dates.
Assay formats used : Enzyme Linked Immuno Sorbent Assay (EIA, ELISA), Competitive ImmunoAssay, Two-site (sandwich) ImmunoAssay, Solid-Phase Immobilized Epitote Immunometric Assay (SPIE-IA)