€577.00
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|Histamine is a natural amine present in many animal and human tissues. It was considered to be the mediator of immediate allergy. Histamine plays a role in various physiological processes, such as control of gastric acid secretion, neurotransmission and modulation of inflammatory and immunological reactions. This competitive enzyme-immunoassay uses a prederivation (derivate reagent with a structure close to the spacer arm used for the immunogen preparation) of histamine that allows to enhance the sensitivity of the assay.
Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
| Size | 96 wells |
|---|---|
| Shipping | dry ice |
| Specificity | 1 methyl histamine: 0.01%, 3 methyl histamine: 0.04%, Histamine: 100%, Histidine: <0.01%, Serotonin: <0.01% |
| Application media | liquid or solid biological samples: extraction HCI, Plasma, urine and culture supernatant: no extraction required, Plasma, urine, culture supernatant as well as liquid (e.g. broncho-alveolar lavage fluids) or solid (brain, nervous tissus) biological samples |
| Sample volume | 100 µL |
| Tracer | AcetylCholinesterase AChE |
| Detection limit | |
| Standard Curve Range | 0.4-50 nM |
| Custom code | 3822000000 |
| UNSPSC code | 41116104 |
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