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Melanocortin-4 Receptor Reporter Assay Kit

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    Melanocortin-<wbr/>4 Receptor Reporter Assay Kit
  • Melanocortin-<wbr/>4 Receptor Reporter Assay Kit
Cat No: 600190
Assays - Miscellaneous
Cayman
€500.00
Price is excluding VAT and does not include packaging neither shipping

Cayman’s MC4R Reporter Assay consist of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). C...

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: 100 tests

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Synonyms:
Product Overview:
Cayman’s MC4R Reporter Assay consist of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.
The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.
Size: 100 tests
Shipping: wet ice
Stability: Store at -20 degrees; shelf life 365 days maximum after production
Custom Code: 3822000002
UNSPSC code: 41116104

MacKenzie, R.G. Obesity-associated mutations in the human melanocortin-4 receptor gene. Peptides 27 395-403 (2006).

Govaerts, C., Srinivasan, S., Shapiro, A., et al. Obesity-associated mutations in the melanocortin 4 receptor provide nov

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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