Growth Hormone (GH) (rat) ELISA

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Growth Hormone (GH) (rat) ELISA kit

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Cat No: A05104

Assay Kits - Elisa

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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                        Territorial Availability: Available worldwide directly through Bertin or your local distributor
                    

                                            Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
                    

Synonyms:

Correlated keywords:

hormones

measures

enzyme

immunoassays

ELISA

spi-bio

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Growth hormone (GH), a polypeptide hormone with a molecular weight of 23000 Daltons released from somatotropes of the anterior pituitary, is regulated by several neurotransmitters and neuropeptides. Among other functions it plays an essential role in regulating body growth.

Size	96 wells
Shipping	Dry ice
Specificity	Rat growth hormone: 100% Mouse growth hormone: 91% Rat prolactin: <1% Rat FSH: <0.1% Human growth hormone: <0.1% Rat LH: <0.1% Rat TSH: <0.1%
Application Media	Plasma and culture supernatant\|No extraction required
Sample volume	50 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	0.04 ng/mL
Standard Curve Range	0.25-25 ng/mL
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: GROWTH HORMONE

Bertherat J., Bluet-Pajot M.T., Epelbaum J. N. Neuroendocrine regulation of growth hormone. Eur J. Endocrinol (1995) Jan;132(1):12-24

Giustina A, Veldhuis JD. Pathophysiology of the neuroregulation of growth hormone secretion in experimental animals and the human. Endocr Rev. 1998 Dec;19(6):717-97

PRODUCT EXPLANATION: GROWTH HORMONE KIT DEVELOPMENT

Ezan E., Laplante E., Bluet-Pajot M.-T. et al. An Enzyme Immunoassay for rat growth hormone: validation and application to the determination of plasma levels and in vitro release. J. Immunoassay, 18(4), 335-336, 1997

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

PRODUCT CITATIONS

Fukuda T., Tanaka T., Hamaguchi Y. et al. Augmented Growth Hormone Secretion and Stat3 Phosphorylation in an Aryl Hydrocarbon Receptor Interacting Protein (AIP)-Disrupted Somatotroph Cell Line. PLoS One. 2016 Oct 5;11(10):e0164131

Pietra C, Takeda Y, Tazawa-Ogata N et al. Anamorelin HCl (ONO-7643), a novel ghrelin receptor agonist, for the treatment of cancer anorexia-cachexia syndrome: preclinical profile. J Cachexia Sarcopenia Muscle. 2014 Dec;5(4):329-37

Domínguez Mancera B, Monjaraz Guzman E, Flores-Hernández JLV et al. Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells. Journal of Biophysics 2013(1):913792, February 2013

Ariyasu H, Iwakura H, Yamada G et al. A postweaning reduction in circulating ghrelin temporarily alters growth hormone (GH) responsiveness to GH-releasing hormone in male mice but does not affect somatic growth. Endocrinology. 2010 Apr;151(4):1743-50.

Lu S.L., Callahan S.M., and Brunner L.J. Suppression of Hepatic CYP3A1/2 and CYP2C11 by Cyclosporine Is Not Mediated by Altering Growth Hormone Levels. J Pharmacol Exp Ther. 2003 Apr;305(1):331-7

Silha J.V., Gui Y., Mishra S. et al. Overexpression of Gly56/Gly80/Gly81-Mutant Insulin-Like Growth Factor-Binding Protein-3 in Transgenic Mice. Endocrinology, March 2005, 146(3):1523–1531

Ma J.N., Schiffer H.H., Knapp A.E. et al. Identification of the Atypical L-Type Ca2+ Channel Blocker Diltiazem and Its Metabolites As Ghrelin Receptor Agonists. Mol Pharmacol. 2007 Aug;72(2):380-6. Epub 2007 May 2

Technical File

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