Anti-Ovalbumin IgE (mouse) ELISA

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Anti-Ovalbumin IgE (mouse) ELISA Kit

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Cat No: 500840

Assay Kits - Elisa

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies pr...

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Correlated keywords:

EIAs ELISAs enzymes immunoassays immunometric kits assays measures measurements immunizations mice mouse with chicken egg albumin OVA precipitate complexes aluminum hydroxides alum TH2-mediated immune immunity response OVA/alum immunized anti-OVA antibody antibodies IgG1 isotypes mediates tissues tissue-specific effectors models chronic inflammation allergy allergic asthma rhinitis cutaneous hypersensitivity levels plasma serum blood immunoglobulin OVA-specific plate-bound biotin-labeled monoclonal antiova ova

Product Overview:
Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic inflammation, including allergic asthma, allergic rhinitis, and cutaneous hypersensitivity. When using one of these models, it is often desirable to measure anti-OVA antibody levels in the plasma or serum to determine the effectiveness of the immunization, the activity of a drug, or the effect of a specific gene deletion. The plasma concentration of OVA-specific IgG1, the predominant anti-OVA immunoglobulin isotype found in the serum or plasma of mice immunized with OVA/alum, is typically 1,000-fold greater than that of OVA-specific IgE. Cayman’s Anti-Ovalbumin IgE (mouse) ELISA Kit is an immunometric assay which can be used to measure anti-ovalbumin of the IgE isotype in mouse plasma and serum without prior sample purification. A plate-bound antibody specific for mouse IgE is used to selectively measure ovalbumin-specific IgE, total plasma or serum IgE, which avoids interference from IgG1 binding. A biotin-labeled ovalbumin reagent is then added to quantify the ovalbumin-specific subset of this captured IgE. A monoclonal anti-ovalbumin IgE produced from mice immunized with OVA/alum is used as the standard. The standard curve spans the range of 1.56-100 ng/ml, with an LLOQ of 3.12 ng/ml.

Size	96 wells
Shipping	wet ice
Custom Code	3822.19
UNSPSC code	41116104

Hogan, S.P., Mould, A., Kikutani, H., et al. Aeroallergen-induced eosinophilic inflammation, lung damage, and airways hyperreactivity in mice can occur independently of IL-4 and allergen-specific immunoglobulins. J Clin Invest 99(6) 1329

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.