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PAD4 (human recombinant)

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    PAD4 (human recombinant)
  • PAD4 (human recombinant)
Cat No: 10500
Cayman
€521.00
Price is excluding VAT and does not include packaging neither shipping

Source: Active recombinant N-terminal His-tagged protein expressed in E. coli • MW: 75.8 kDa • PAD4 is a homodimer that functions as a transcriptional coregulator to catalyze the conversion of specific arginine residues to citrulline in a calcium-de...

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: 100 µg

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Product Overview:
Source: Active recombinant N-terminal His-tagged protein expressed in E. coli • MW: 75.8 kDa • PAD4 is a homodimer that functions as a transcriptional coregulator to catalyze the conversion of specific arginine residues to citrulline in a calcium-dependent manner. PAD4 substrates include histones H2A, H3, and H4, whose post-translational modifications play a large role in gene regulation.
Protein Arginine Deiminases (PADs) are guanidino-modifying enzymes belonging to the amidinotransferase superfamily and are designated PAD1-4 and PAD6. All enzymes are cytosolic except for PAD4 which is localized in the nucleus. PAD4 is a homodimer that functions as a transcriptional coregulator to catalyze the conversion of specific arginine residues to citrulline in a calcium-dependent manner. PAD4 substrates include histones H2A, H3, and H4, whose post-translational modifications play a large role in gene regulation. Benzoylated arginine substrates like N-α-Benzoyl-L-arginine ethyl ester (BAEE) have proven to be useful tools for characterization of PAD4, having similar kinetic properties to the natural substrates. PAD4 itself can undergo autocitrullination at several sites, which inhibits its enzymatic activity and may play an important role in regulating citrullination in cells. PAD4 activity is increased in rheumatoid arthritis, producing an abundance of citrulline-containing proteins that can be recognized by autoantibodies, which cause the immune system to attack its own tissues. PAD4 has also been implicated in several other diseases including multiple sclerosis, Alzheimer’s disease, glaucoma, and cancer.
Size: 100 µg
Shipping: dry ice
Stability: Store at -80 degrees; shelf life 365 days maximum after production
Formulation: 50 mM Tris, pH 7.6, containing 500 mM sodium chloride, 1 mM DTT, and 0.5 mM EDTA
Purity: ≥80% estimated by SDS-PAGE
Custom Code: 3507907000
UNSPSC code: 12352204

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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