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Alanine Transaminase Colorimetric Activity Assay Kit

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    Alanine Transaminase Colorimetric Activity Assay Kit
  • Alanine Transaminase Colorimetric Activity Assay Kit
Cat No: 700260
Assays - Enzymatic Kits
Cayman
€251.00
Price is excluding VAT and does not include packaging neither shipping

ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity...

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: 96 wells

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Special Advice: This kit has multiple shipping temperatures
Product Overview:
ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates. Measurement of the ALT activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing LDH. The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm.
Alanine Transaminase (ALT), also known as alanine aminotransferase (ALAT) or serum glutamic pyruvic transaminase (sGPT), is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. ALT mediates conversion of major intermediate metabolites, catalyzing reversible transamination between alanine and α-ketoglutarate to form pyruvate and glutamate. ALT is widely distributed in many tissues but is found in greatest abundance in the liver, and to a much lesser extent in the kidneys, heart, and brain. The major role of ALT in the liver is the conversion of alanine to glucose which is then exported to the body to be utilized in a multitude of processes. ALT has also been found to play an important role in neuronal function by supplying an important source of neuronal glutamate through the analine-aminotransferase reaction. Serum ALT levels are generally low, but may spike during disease states or in the event of tissue injury. As such, ALT levels are routinely used as indicators of medical issues, particularly liver diseases. Increased levels can be seen in patients with diabetes, cirrhosis, fatty liver disease, and hepatitis. Beyond liver disease, increased ALT levels have been noted in cases of carcinoma, mononucleosis, muscular dystrophy, and cardiovascular disease. Cayman’s Alanine Transaminase Colorimetric Assay Kit provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and cell lysates. Measurement of the ALT activity is carried out by monitoring the rate of NADH oxidation in a coupled reaction system employing lactate dehydrogenase (LDH). The oxidation of NADH to NAD+ is accompanied by a decrease in absorbance at 340 nm. Under circumstances in which the ALT activity is rate limiting, the rate decrease is directly proportional to the ALT activity in the sample.
Size: 96 wells
Shipping: dry ice
Stability: Store at -80 degrees; shelf life 365 days maximum after production
Custom Code: 3822000002
UNSPSC code: 41116104

Yang, R., Park, S., Reagan, W.J., et al. Alanine aminotransferase isoenzymes: Molecular cloning and quantitative analysis of tissue expression in rats and serum elevation in liver toxicity. Hepatology 49 598-607 (2009).

Pratt, D.S., and Kaplan, M.M. Evaluation of abnormal liver-enzyme results in asymptomatic patients. N Engl J Med 342(17) 1266-1271 (2000).

Vozarova, B., Stefan, N., Lindsay, R.S., et al. High alanine aminotransferase is associated with decreased hepatic insulin sensitivity and predicts the development of type 2 diabetes. Diabetes 51 1889-1895 (2002).

Kim, H.C., Nam, C.M., Jee, S.H., et al. Normal serum aminotransferase concentration and risk of mortality from liver diseases: Prospective cohort study. BMJ (2004).

Prati, D., Taioli, E., Zanella, A., et al. Updated definitions of healthy ranges for serum alanine aminotransferase levels. Ann Intern Med 137 1-9 (2002).

Ishiguro, M., Takio, K., Suzuki, M., et al. Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods. Biochemistry 30 10451-10457 (1991).

Erecinska, M., Nelson, D., Nissim, I., et al. Cerebral alanine transport and alanine aminotransferase reaction: Alanine as a source of neuronal glutamate. J Neurochem 62 1953-1964 (1994).

Tarao, K., Rino, Y., Ohkawa, S., et al. Association between high serum alanine aminotransferase levels and more rapid development and higher rate of incidence of hepatocellular carcinoma in patients with hepatitis C virus-associated cirrhosis. Cancer 86 589-595 (1999).

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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