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Cholesterol Uptake Cell-Based Assay Kit

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    Cholesterol Uptake Cell-<wbr/>Based Assay Kit
  • Cholesterol Uptake Cell-<wbr/>Based Assay Kit
Cat No: 600440
Assays - Other Eia Kits
Cayman
€244.00
Price is excluding VAT and does not include packaging neither shipping

Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. The mechanisms controlling cho...

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: 1 ea

This product can only be bought through Cayman Chemical. Please contact us.

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Territorial Availability: Available through Bertin Technologies only in Europe
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. The mechanisms controlling cholesterol absorption are important to understand for the prevention and treatment of hypercholesterolemia. Cayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently-tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, a cholesterol intracellular trafficking inhibitor, is included as a positive control.
Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. However, the mechanisms controlling cholesterol absorption are not well understood. A better understanding of the relationship between cholesterol absorption and synthesis may allow for the development of powerful novel strategies for the prevention and treatment of hypercholesterolemia. Cayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently-tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, a cholesterol intracellular trafficking inhibitor, is included as a positive control.
Size: 1 ea
Shipping: wet ice
Stability: Store at -20 degrees; shelf life 180 days maximum after production
Custom Code: 3822000002
UNSPSC code: 41116104
UN Number: UN1170

Wang, D.Q. Regulation of intestinal cholesterol absorption. Annu Rev Physiol 69 221-248 (2011).

Altmann, S.W., Davis, H.R., Zhu, L., et al. Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption. Science 303 1201-1204 (2004).

Soccio, R.E., and Breslow, J.L. Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24 1150-1160 (2004).

Santosa, S., Varady, K.A., AbuMweis, S., et al. Physiological and therapeutic factors affecting cholesterol metabolism: Does a reciprocal relationship between cholesterol absorption and synthesis really exist. Life Sci 80(6) 505-514 (2007).

Davis, H.R., Zhu, L., Hoos, L.M., et al. Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. J Biol Chem 279(32) 33586-33592 (2004).

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.

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