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Glyco-SPOT DNA Repair Assay Kit

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    Glyco-SPOT DNA Repair Assay Kit
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    Glyco-SPOT DNA Repair Assay Kit
  • Glyco-SPOT DNA Repair Assay Kit
  • Glyco-SPOT DNA Repair Assay Kit
Cat No: LX05001
Assays - Miscellaneous
SPOT-LX
€1,200.00
Price is excluding VAT and does not include packaging neither shipping

Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases...

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: 24 wells
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Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Product Overview:
Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases followed by the processing of the resulting abasic site either by the AP-lyase activity of the bifunctional glycosylase or by the apurinic/apyrimidic endonuclease APE1 that incises the DNA strand. BER mechanisms can be induced by oxidative stress and various genotoxic attacks.
The Glyco-SPOT DNA Repair Assay is a multiplexed oligonucleotide (ODN) cleavage assay developed on support. It is used to monitor simultaneously the cleavage efficiency of several glycosylases/AP endonucleases against a set of emblematic DNA lesions in cell/bacteria/tissue extracts, using fluorescent detection.
The assay can be used to screen for DNA repair inhibitors, characterize DNA repair enzymatic signature from samples, check DNA glycosylase specificity, characterize DNA repair inhibitory properties of chemicals (heavy metals …).
Size: 24 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6 months maximum after production
Application Media: The assay can be conducted with whole cell extracts, nuclear extracts, tissue extracts or purified enzymes.
Custom Code: 38220000
UNSPSC code: 41116104

Garreau-Balandier I, et al. A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray. FEBS Lett. 2014; 588:1673-9.

Candéias S, et al. Direct inhibition of excision/synthesis DNA repair activities by cadmium: analysis on dedicated Biochips. Mutat Res. 2010; 10:53-9

Pons B, et al. Age-associated modifi cations of Base Excision Repair activities in human skin fi broblast extracts. Mech Ageing Dev. 2010;131:661-5

Guerniou V, et al. Repair of oxidative damage of thymine by HeLa whole-cell extracts: simultaneous analysis using a microsupport and comparison with traditional PAGE analysis. Biochimie. 2005; 87:151-9.

Sauvaigo S, et al. An oligonucleotide microarray for the monitoring of repair enzyme activity toward different DNA base damage. Anal Biochem. 2004; 333:182-92

SPOT-LX is based on a technology powered by LXRepair – spin-off of the CEA (French Alternative Energies and Atomic Energy Commission). This outstanding technology addresses the need to understand and characterize the DNA Repair network, a major cellular defence mechanisms against DNA damage. SPOT-LX assay allows defining a DNA Repair Enzyme Signature that quantify the functionality of various DNA Repair Enzymes.

DNA Repair enzymes contained in a biological sample react with series of lesion-containing DNA on a biochip. The repair reaction occurring at each lesion site is measured by fluorescence analysis.

Bertin Bioreagent offers pharmaceutical and cosmetic companies a tool for characterizing mechanisms of action of their molecules including the stages of research and clinical studies with an assay ready for use: the range of SPOT-LX assay kit.

Applications also include study of ageing, neurological diseases, oxidative stress, inflammation and exposure to genotoxics to identify specific biomarkers related to DNA Repair.

This DNA Repair Assay kit is based on multiplexed in vitro patented technologies (CEA and UJF under license) that measure DNA repair in micro-holder.

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