Territorial Availability: Available worldwide directly through Bertin or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Resistin is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Previous studies have shown that in mice, resistin impairs glucose tolerance and insulin action and inhibits adipogenesis in murine 3T3-L1 cells. Therefore, resistin has also been proposed as an adipocyte secreted factor that is thought to link obesity and type 2 diabetes. The role of resistin in human obesity remains controversial even if some data show that Thiazolinedione down regulate resitin.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 9 months maximum after production
Application Media: Human serum, plasma and culture supernatant|1/3 dilution for serum and plasma prior assay
Sample volume: 50 µL
Detection Limit: 0,1 ng/mL
Standard Curve Range: 1-50 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104