Nrf2 Transcription Factor Assay

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Nrf2 Transcription Factor Assay Kit

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Cat No: 600590

Assay Kits - Elisa

Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein Kelch-like ECH-associated protein 1 (Keap1). In resting cells, Nrf2 and Keap1 f...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

assays kits measuring measurement TFs factors NF-E2-related 2 Nuclear (erythroid-derived 2)-like 2 NFE2L2 E2 related leucines zippers types zipper-types transcriptions ubiquitously expressed expressions kidneys muscles lungs livers redox homeostasis oxidations reductions interactions cysteines cysteine-rich proteins Kelch like Kelch-like ECH-associated ECH associated 1 Keap1 resting cells complexes targets targeted targeting degrades degradations proteasomes oxidatives stresses undergoes conformational changes phosphoryateds phosphorylations releases releasing Nrf2/Keap1 translocations nucleus nuclei induced inductions genes encodes encoding cytoprotections cytoprotectives antioxidatives antioxidants enzymes activates activations defenses systems cellular molecules molecular damages cancers developments diseases diabetes neurodegenerative neurodegenerations diets dietary factors curcumin downstream lowers lowering inflammations inhibitors inhibits inhibitions carcinogenesis protections xenobiotics drugs interventions disorders nonradioactives non-radioactives non radioactives methods specifics DNA deoxyribonucleic acids binds bindings activity activities nuclear extracts double stranded dsDNA response elements primary antibody antibodies secondary 1 2 HRP horseradish peroxidases colorimetric ELISA EMSA enzyme-linked immunosorbents assays immunoassays radioactives electrophoretics mobility shift genes regulation

Product Overview:
Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein Kelch-like ECH-associated protein 1 (Keap1). In resting cells, Nrf2 and Keap1 form a tight complex, which is targeted for degradation by proteasomes. Under oxidative stress, Nrf2 is released from the Nrf2/Keap1 complex and translocates to the nucleus where it is able to induce the expression of a battery of genes encoding diverse cytoprotective proteins, including antioxidative enzymes, anti-inflammatory mediators, and proteasomes. Cayman’s Nrf2 Transcription Factor Assay is a non-radioactive, colorimetric method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the Nrf2 response element is immobilized onto the wells of a 96-well plate. Nrf2 contained in nuclear extract samples will bind specifically to the Nrf2 response element and can be detected by addition of a specific Nrf2 antibody and a secondary antibody conjugated to HRP.

Size	96 wells
Shipping	dry ice
Custom Code	3822.19
UNSPSC code	41116104

Surh, Y., Kundu, J.K., and Na, H. Nrf2 as a master redox switch in turning on the cellular signaling involved in the induction of cytoprotective genes by some chemopreventive phytochemicals. Planta Med 74 1526-1539 (2008).

Taguchi, K., Motohashi, H., and

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.