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Insulinase Inhibitor Cocktail

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    Insulinase Inhibitor Cocktail
  • Insulinase Inhibitor Cocktail
Cat No: D05011
Pre-Analytical Products - Sample Collection
SPI-Bio
€341.00
Price is excluding VAT and does not include packaging neither shipping

The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for the past few years, (c) the reactivity (or lack of reactivity) of rapid- or long-acting pharmacological ins...

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: 500 µl
- +
Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Product Overview:
The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for the past few years, (c) the reactivity (or lack of reactivity) of rapid- or long-acting pharmacological insulin analogs in sera (1 ). Hemolyzed samples contain an insulin-degrading enzyme and should not be analyzed unless they can be handled at 4 °C within 2 to 3 h or an insulinase inhibitor cocktail has been added in the blood collection tube to prevent insulin degradation.
This Inhibitor cocktail has been developed and validated with Bertin Bioreagent Insulin (mouse, rat) EIA kit #A05105.
Size: 500 µl
Shipping: dry ice
Stability: Store at -20degrees; shelf life 1 year maximum after production
Application(s):

This protocol may be used to measure insulin in plasma or serum sample where hemolysis may occur. To do so, blood samples are collected in tubes containing EDTA. The samples are centrifuged at 1600 g for 20 minutes. Plasmas are collected and kept at -20°C until assay.

Allow to collect 2.5 mL of sample and process one kit.

Thaw inhibitor cocktail #D05011 and add 10 µL for 1mL of plasma (or 5 µL for 500 µL, 2 µL for 200 µLetc..).

Add 10µL of inhibitor cocktail #D05011 in the reconstituted Insulin Standard ( vial #A06105 reconstituted with 1mL UltraPure water), and 10µL of inhibitor cocktail in the reconstituted Insulin QC ( vial #A10105 reconstituted with 1mL UltraPure water).

Inhibitor Buffer will be used to process additional dilutions : serial dilutions of the Insulin Standard, dilutions of the samples. How to prepare Inhibitor Buffer : prepare EIA Buffer by diluting the vial #A07000 with 50 mL UltraPure water; pipet 25 mL of this EIA Buffer and add 250 µL of the inhibitor cocktail #D05011. Mix thoroughly by gentle inversion.

Custom Code: 3822000000
UNSPSC code: 12352100

Chevenne D., Letailleur A., Trivin F., and Porquet D.,Effect of Hemolysis on the Concentration of Insulin in Serum Determined by RIA and IRMA, Clin. Chem., 44: 354 - 356, Feb 1998

Sapin R., Insulin Immunoassays: Fast Approaching 50 Years of Existence and Still Calling for Standardization, Clinical Chemistry 53: 810-812, 2007

SPI-Bio is a brand name of Bertin Bioreagent.
Bertin Bioreagent includes over 100 employees, chemists, biochemists, immunoanalysts, pharmacists and technicians.
Always at the forefront of innovation and after launching on the market products incorporating in their time innovative technologies (Acetylcholinesterase tracer for EIA), this brand offers a wide and unique range of assays for biomarker Immunoassays (SPI-Bio TM) thanks to a solid knowledge acquired during the last 25 years in developping over 100 biomarkers assays.

To better serve you, we aim to design our products and reagents to address your needs on the expected performance in terms of a possible range of use, but also on operational conditions of use & regulations.
Thanks to our production processes and quality controls, we provide you with products with compliance to specifications.
Our technical support & science teams are available to help you assess their relevance depending on your needs, to specify the conditions of application and their fields of use. Finally, our researchers are ready to provide you with the necessary support by going, if needed, in your laboratories to validate your operating conditions and to solve possible problems.

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